Clone: HsCD00295123

Clone ID:HsCD00295123
Is Verified:Y
Status:AVAILABLE
Source:The ORFeome Collaboration
Description:
Comments:
Type:cDNA
Verification Method:Sequence Verification
Distribution:No restriction
Special MTA:
Map:

Related Identifiers:

IMAGE ID 100068216
Original Clone ID FLH481378.01X
GI 189442866
HIP Clone ID 481378
GenBank Accession BC167826
HIP Master Clone ID 399106

Property:

Collection: NIH_MGC_435
Collection: OCAB23
Collection: The ORFeome Collaboration

Insert Information:

Insert Size (bp) Species Format Tissue Source Species Specific ID Gene Symbol Gene Name Keyword
1 1578 Homo sapiens CLOSED 64714 PDIA2 protein disulfide isomerase family A, member 2
Insert1
Size (bp)1578
SpeciesHomo sapiens
FormatCLOSED
Tissue Source
Species Specific ID 64714
Gene SymbolPDIA2
Gene Nameprotein disulfide isomerase family A, member 2
Keyword

Insert Sequence:

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAGCCGCCAGCTTCTGCCTGTACTGCTGCTGCTGCTGCTCAGGGCTTCGTGCCCATGGGGTCAGGAACAGGGAGCGAGGAGCCCCTCGGAGGAGCCTCCAGAGGAGGAAATCCCCAAGGAGGATGGGATCTTGGTGCTGAGCCGCCACACCCTGGGCCTGGCCCTGCGGGAGCACCCTGCCCTGCTGGTGGAATTCTATGCCCCGTGGTGTGGGCACTGCCAGGCCCTGGCCCCCGAGTACAGCAAGGCAGCTGCCGTGCTCGCGGCCGAGTCAATGGTGGTCACGCTGGCCAAGGTGGATGGGCCCGCGCAGCGCGAGCTGGCTGAGGAGTTTGGTGTGACGGAGTACCCTACGCTCAAGTTCTTCCGCAATGGGAACCGCACGCACCCGGAGGAGTACACAGGACCACGGGACGCTGAGGGCATTGCCGAGTGGCTGCGACGGCGGGTGGGGCCCAGTGCCATGCGGCTGGAGGACGAGGCGGCCGCCCAGGCGCTGATCGGTGGCCGGGACCTAGTGGTCATTGGCTTCTTCCAGGACCTGCAGGACGAGGACGTGGCCACCTTCTTGGCCTTGGCCCAGGACGCCCTGGACATGACCTTTGGCCTCACAGACCGGCCGCGGCTCTTTCAGCAGTTTGGCCTCACCAAGGACACTGTGGTTCTCTTCAAGAAGTTTGATGAGGGGCGGGCAGACTTCCCCGTGGACGAGGAGCTTGGCCTGGACCTGGGGGATCTGTCGCGCTTCCTGGTCACACACAGCATGCGCCTGGTCACGGAGTTCAACAGCCAGACGTCTGCCAAGATCTTCGCGGCCAGGATCCTCAACCACCTGCTGCTGTTTGTCAACCAGACGCTGGCTGCGCACCGGGAGCTCCTAGCGGGCTTTGGGGAGGCAGCTCCCCGCTTCCGGGGGCAGGTGCTGTTCGTGGTGGTGGACGTGGCGGCCGACAATGAGCACGTGCTGCAGTACTTTGGACTCAAGGCTGAGGCAGCCCCCACTCTGCGCTTGGTCAACCTTGAAACCACTAAGAAGTATGCGCCTGTGGATGGGGGCCCTGTCACCGCAGCGTCCATCACTGCTTTCTGCCATGCAGTCCTCAACGGCCAAGTCAAGCCCTATCTCCTGAGCCAGGAGATACCCCCTGATTGGGATCAGCGGCCAGTTAAGACCCTCGTGGGCAAGAATTTTGAGCAGGTGGCTTTTGACGAAACCAAGAATGTGTTTGTCAAGTTCTATGCCCCGTGGTGCACCCACTGCAAGGAGATGGCCCCTGCCTGGGAGGCATTGGCTGAGAAGTACCAAGACCACGAGGACATCATCATTGCTGAGCTGGATGCCACGGCCAACGAGCTGGATGCCTTCGCTGTGCACGGCTTCCCTACTCTCAAGTACTTCCCAGCAGGGCCAGGTCGGAAGGTGATTGAATACAAAAGCACCAGGGACCTGGAGACTTTCTCCAAGTTCCTGGACAACGGGGGCGTGCTGCCCACGGAGGAGCCCCCGGAGGAGCCAGCAGCCCCGTTCCCGGAGCCACCGGCCAACTCCACTATGGGGTCCAAGGAGGAACTGTAGGGCCTCATGGGCCCAGCTTTCTTGTAC

Insert Property: Insert 1

Type Value Extra Information
End on reference sequence 1630
Start on reference sequence 53

Inferred Whole Plasmid Sequence: (BETA)
Insert green, Vector black

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAGCCGCCAGCTTCTGCCTGTACTGCTGCTGCTGCTGCTCAGGGCTTCGTGCCCATGGGGTCAGGAACAGGGAGCGAGGAGCCCCTCGGAGGAGCCTCCAGAGGAGGAAATCCCCAAGGAGGATGGGATCTTGGTGCTGAGCCGCCACACCCTGGGCCTGGCCCTGCGGGAGCACCCTGCCCTGCTGGTGGAATTCTATGCCCCGTGGTGTGGGCACTGCCAGGCCCTGGCCCCCGAGTACAGCAAGGCAGCTGCCGTGCTCGCGGCCGAGTCAATGGTGGTCACGCTGGCCAAGGTGGATGGGCCCGCGCAGCGCGAGCTGGCTGAGGAGTTTGGTGTGACGGAGTACCCTACGCTCAAGTTCTTCCGCAATGGGAACCGCACGCACCCGGAGGAGTACACAGGACCACGGGACGCTGAGGGCATTGCCGAGTGGCTGCGACGGCGGGTGGGGCCCAGTGCCATGCGGCTGGAGGACGAGGCGGCCGCCCAGGCGCTGATCGGTGGCCGGGACCTAGTGGTCATTGGCTTCTTCCAGGACCTGCAGGACGAGGACGTGGCCACCTTCTTGGCCTTGGCCCAGGACGCCCTGGACATGACCTTTGGCCTCACAGACCGGCCGCGGCTCTTTCAGCAGTTTGGCCTCACCAAGGACACTGTGGTTCTCTTCAAGAAGTTTGATGAGGGGCGGGCAGACTTCCCCGTGGACGAGGAGCTTGGCCTGGACCTGGGGGATCTGTCGCGCTTCCTGGTCACACACAGCATGCGCCTGGTCACGGAGTTCAACAGCCAGACGTCTGCCAAGATCTTCGCGGCCAGGATCCTCAACCACCTGCTGCTGTTTGTCAACCAGACGCTGGCTGCGCACCGGGAGCTCCTAGCGGGCTTTGGGGAGGCAGCTCCCCGCTTCCGGGGGCAGGTGCTGTTCGTGGTGGTGGACGTGGCGGCCGACAATGAGCACGTGCTGCAGTACTTTGGACTCAAGGCTGAGGCAGCCCCCACTCTGCGCTTGGTCAACCTTGAAACCACTAAGAAGTATGCGCCTGTGGATGGGGGCCCTGTCACCGCAGCGTCCATCACTGCTTTCTGCCATGCAGTCCTCAACGGCCAAGTCAAGCCCTATCTCCTGAGCCAGGAGATACCCCCTGATTGGGATCAGCGGCCAGTTAAGACCCTCGTGGGCAAGAATTTTGAGCAGGTGGCTTTTGACGAAACCAAGAATGTGTTTGTCAAGTTCTATGCCCCGTGGTGCACCCACTGCAAGGAGATGGCCCCTGCCTGGGAGGCATTGGCTGAGAAGTACCAAGACCACGAGGACATCATCATTGCTGAGCTGGATGCCACGGCCAACGAGCTGGATGCCTTCGCTGTGCACGGCTTCCCTACTCTCAAGTACTTCCCAGCAGGGCCAGGTCGGAAGGTGATTGAATACAAAAGCACCAGGGACCTGGAGACTTTCTCCAAGTTCCTGGACAACGGGGGCGTGCTGCCCACGGAGGAGCCCCCGGAGGAGCCAGCAGCCCCGTTCCCGGAGCCACCGGCCAACTCCACTATGGGGTCCAAGGAGGAACTGTAGGGCCTCATGGGCCCAGCTTTCTTGTACTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Sequencing Information:

Phred Score: 0
Date: 2013-03-29

Last Passing Sequencing Read:

TGATGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAGCCGCCAGCTTCTGCCTGTACTGCTGCTGCTGCTGCTCAGGGCTTCGTGCCCATGGGGTCAGGAACAGGGAGCGAGGAGCCCCTCGGAGGAGCCTCCAGAGGAGGAAATCCCCAAGGAGGATGGGATCTTGGTGCTGAGCCGCCACACCCTGGGCCTGGCCCTGCGGGAGCACCCTGCCCTGCTGGTGGAATTCTATGCCCCGTGGTGTGGGCACTGCCAGGCCCTGGCCCCCGAGTACAGCAAGGCAGCTGCCGTGCTCGCGGCCGAGTCAATGGTGGTCACGCTGGCCAAGGTGGATGGGCCCGCGCAGCGCGAGCTGGCTGAGGAGTTTGGTGTGACGGAGTACCCTACGCTCAAGTTCTTCCGCAATGGGAACCGCACGCACCCGGAGGAGTACACAGGACCACGGGACGCTGAGGGCATTGCCGAGTGGCTGCGACGGCGGGTGGGGCCCAGTGCCATGCGGCTGGAGGACGAGGCGGCCGCCCAGGCGCTGATCGGTGGCCGGGACCTAGTGGTCATTGGCTTCTTCCAGGACCTGCAGGACGAGGACGTGGCCACCTTCTTGGCCTTGGCCCAGGACGCCCTGGACATGACCTTTGGCCTCACAGACCGGCCGCGGCTCTTTCAGCAGTTTGGCCTCACCAAGGACACTGTGGTTCTCTTCAAGAAGTTTGATGAGGGGCGGGCAGACTTCCCCGTGGACGAGGAGCTTGGCCTGGACCTGGGGGATCTGTCGCGCTTCCTGGTCACACACAGCATGCGCCTGGTCACGGAGTTCAACAGCCAGACGTCTGCCAAGATCTTCGCGGCCAGGATCC

Vector Information:

Vector Name:pENTR223.1
Type:bacterial plasmid
Description:Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Properties:Gateway, donor (entry), recombinational cloning
Comments:Additional information about this vector is available from the commercial website and at the ORFeome website http://www.orfeomecollaboration.org/html/vectors.shtml.
Map:pENTR223-1-map.doc
Sequence:pENTR223_1.txt
Size (bp):4311
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha T1 phage resistant Y

Antibiotic Selections:

Host Type Marker
bacterial spectinomycin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL spectinomycin Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Authors:

Author Name Author Type
The ORFeome Collaboration Consortium