Clone: EvNO00292861
| Clone ID: | EvNO00292861 |
| Is Verified: | N |
| Status: | AVAILABLE |
| Source: | HIP |
| Description: | Lentiviral cDNA expression vector, with C-terminal 6xHis tag and N-terminal HA-Flag tag separated by a TEV protease cleavage site; Ampicillin and chloromphenical resistance in bacteria; blasticidin resistance in mammalian cells; CRE-Lox recombinational or restriction enzyme cloning. |
| Comments: | |
| Type: | No insert |
| Verification Method: | |
| Distribution: | No restriction |
| Special MTA: |
| Map: | pCW62-Map.pdf |
Related Identifiers:
Sequencing Information:
| Phred Score: | 777 |
| Date: | 2018-03-19 |
Last Passing Sequencing Read:
NNCCCTCGTCAGCATTACANACTCGTTAGTGACCGTCAGATCGCCTGGAG ACGCCATCCACGCTGATTTTGACCTCTATAGAAGACACCGGGACCGATCC AGCCTCCGGCTAGCCATAATACGACTCACTATAGGGTTTAAACTTAAGAC ACCATGTACCCCTACGACGTGCCCGACTACGCCGAAAACCTATACTTCCA ATCACTGCAGGACTACAAGGACGACGATGACAAGGTCGACATAACTTCGT ATAGCATACATTATACGAAGTTATAGATCCAATATTATTGAAGCATTTAT CAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAA TAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACG TGGATCTCGAGCTCAAGCTTCGAATTCAGGGTTTCCTTGACAATATCATA CTTATCCTGTCCCTTTTTTTTCCACAGCTACCGGTCCACCACCACCACCA CCACTAACCCGGGGGATCCGCGGCCGCAAATTCCGCCCCTCTCCCTCCCC CCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGT TTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGG GCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTC CCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAG TTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGC AGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCC ACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCCCGTTG TGAATTGGATAGTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCA CAAGGGGCTGAAGGATGCCCAAAAGGTACCCCATTGTAGGGGATCTGATC GGGGGCCCCGGGGCCAATGCTTTACTGGGGTTAACCGAGGTTAAAAAAAC GTTTGGGCCCCCCGAAACACGGGAACGTGGTTTCCCTTTAAAAACCCGAG ATAAGCTTGCCCAACATGGCCAAACCTTTGCCCCAAAAAAACCCCCTCCT TGAAGAAAAAGGGTACAATCAAAGATTTCCCTTTTTGAAAATTAAGGTTC CGGGGGATTTCTTATAGAGGCGGGCTTTCGGGGGGAAGGATATCTTTTAC GGGGGAACTGGTGAAACCGGGGGTGTGGACTGGTGGTGTGGGAACTGAAA CTTATTTTTTCCCATTAGAATGAAAAGGGATTTGACCCGGGAAGGGCAAA GGTTTCTATTTCCCGGTTAAACCTGTAAGAGTGGACACCCCGCTGTGTTT AATTCCCCGTTGTGGGGAAAATTACTAATAGTCTTTCTGGAACCTTTACT
Vector Information:
| Vector Name: | pCW62 |
| Type: | bacterial plasmid |
| Description: | Lentiviral cDNA expression vector, with C-terminal 6xHis tag and N-terminal HA-Flag tag separated by a TEV protease cleavage site; Ampicillin and chloromphenical resistance in bacteria; blasticidin resistance in mammalian cells; CRE-Lox recombinational or restriction enzyme cloning. |
| Properties: | mammalian expression |
| Comments: | |
| Map: | pCW62-Map.pdf |
| Sequence: | pCW62-DNA-Sequence.dat |
| Size (bp): | 7520 |
| Form: | dsDNA |
Host Information:
| Host Strain | Is Used In Distribution | Description |
| DH5-alpha T1 phage resistant | Y | T1/T5 phage resistant DH5-alpha |
Antibiotic Selections:
| Host Type | Marker |
| bacterial | ampicillin |
| bacterial | chloramphenicol |
| mammalian | blasticidin |
Recommended Growth Condition:
| Host Type | Selection Condition | Growth Condition | Comments |
| bacterial | Ampicillin, Chloramphenicol | 50 ug/ml ampicillin or carbenicillin, 25 ug/ml chloromphenical and 7 % sucrose | Use this for CRE-lox cloning media and for clones containing inserts |
