Clone ID:	EvNO00292861
Is Verified:	N
Status:	AVAILABLE
Source:	HIP
Description:	Lentiviral cDNA expression vector, with C-terminal 6xHis tag and N-terminal HA-Flag tag separated by a TEV protease cleavage site; Ampicillin and chloromphenical resistance in bacteria; blasticidin resistance in mammalian cells; CRE-Lox recombinational or restriction enzyme cloning.
Comments:
Type:	No insert
Verification Method:
Distribution:	No restriction
Special MTA:

Map:	pCW62-Map.pdf

Related Identifiers:

Sequencing Information:

Phred Score:	777
Date:	2018-03-19

Last Passing Sequencing Read:

NNCCCTCGTCAGCATTACANACTCGTTAGTGACCGTCAGATCGCCTGGAG ACGCCATCCACGCTGATTTTGACCTCTATAGAAGACACCGGGACCGATCC AGCCTCCGGCTAGCCATAATACGACTCACTATAGGGTTTAAACTTAAGAC ACCATGTACCCCTACGACGTGCCCGACTACGCCGAAAACCTATACTTCCA ATCACTGCAGGACTACAAGGACGACGATGACAAGGTCGACATAACTTCGT ATAGCATACATTATACGAAGTTATAGATCCAATATTATTGAAGCATTTAT CAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAA TAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACG TGGATCTCGAGCTCAAGCTTCGAATTCAGGGTTTCCTTGACAATATCATA CTTATCCTGTCCCTTTTTTTTCCACAGCTACCGGTCCACCACCACCACCA CCACTAACCCGGGGGATCCGCGGCCGCAAATTCCGCCCCTCTCCCTCCCC CCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGT TTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGG GCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTC CCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAG TTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGC AGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCC ACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCCCGTTG TGAATTGGATAGTGTGGAAAGAGTCAAATGGCTCTCCTCAAGCGTATTCA CAAGGGGCTGAAGGATGCCCAAAAGGTACCCCATTGTAGGGGATCTGATC GGGGGCCCCGGGGCCAATGCTTTACTGGGGTTAACCGAGGTTAAAAAAAC GTTTGGGCCCCCCGAAACACGGGAACGTGGTTTCCCTTTAAAAACCCGAG ATAAGCTTGCCCAACATGGCCAAACCTTTGCCCCAAAAAAACCCCCTCCT TGAAGAAAAAGGGTACAATCAAAGATTTCCCTTTTTGAAAATTAAGGTTC CGGGGGATTTCTTATAGAGGCGGGCTTTCGGGGGGAAGGATATCTTTTAC GGGGGAACTGGTGAAACCGGGGGTGTGGACTGGTGGTGTGGGAACTGAAA CTTATTTTTTCCCATTAGAATGAAAAGGGATTTGACCCGGGAAGGGCAAA GGTTTCTATTTCCCGGTTAAACCTGTAAGAGTGGACACCCCGCTGTGTTT AATTCCCCGTTGTGGGGAAAATTACTAATAGTCTTTCTGGAACCTTTACT

Vector Information:

Vector Name:	pCW62
Type:	bacterial plasmid
Description:	Lentiviral cDNA expression vector, with C-terminal 6xHis tag and N-terminal HA-Flag tag separated by a TEV protease cleavage site; Ampicillin and chloromphenical resistance in bacteria; blasticidin resistance in mammalian cells; CRE-Lox recombinational or restriction enzyme cloning.
Properties:	mammalian expression
Comments:
Map:	pCW62-Map.pdf
Sequence:	pCW62-DNA-Sequence.dat
Size (bp):	7520
Form:	dsDNA

Host Information:

Host Strain	Is Used In Distribution	Description
DH5-alpha T1 phage resistant	Y	T1/T5 phage resistant DH5-alpha

Antibiotic Selections:

Host Type	Marker
bacterial	ampicillin
bacterial	chloramphenicol
mammalian	blasticidin

Recommended Growth Condition:

Host Type	Selection Condition	Growth Condition	Comments
bacterial	Ampicillin, Chloramphenicol	50 ug/ml ampicillin or carbenicillin, 25 ug/ml chloromphenical and 7 % sucrose	Use this for CRE-lox cloning media and for clones containing inserts