Clone: HsCD00288356

Clone ID:HsCD00288356
Is Verified:Y
Status:AVAILABLE
Source:The ORFeome Collaboration
Description:
Comments:
Type:cDNA
Verification Method:Sequence Verification
Distribution:No restriction
Special MTA:
Map:

Related Identifiers:

HIP Clone ID 471593
HIP Master Clone ID 394511
Original Clone ID 29946

Property:

Collection: The ORFeome Collaboration

Insert Information:

Insert Size (bp) Species Format Tissue Source Species Specific ID Gene Symbol Gene Name Keyword
1 945 Homo sapiens FUSION 51011 FAHD2A fumarylacetoacetate hydrolase domain containing 2A
Insert1
Size (bp)945
SpeciesHomo sapiens
FormatFUSION
Tissue Source
Species Specific ID 51011
Gene SymbolFAHD2A
Gene Namefumarylacetoacetate hydrolase domain containing 2A
Keyword

Insert Sequence:

ATGCTGGTGTCTGGTAGAAGAAGGTTACTCACAGTTCTGCTGCAGGCTCAGAAGTGGCCCTTTCAACCCTCCAGAGACATGAGACTAGTGCAGTTCCGGGCACCCCACCTGGTGGGGCCTCACTTGGGCCTGGAGACAGGGAATGGTGGAGGGGTTATCAACCTCAATGCCTTTGACCCCACACTCCCGAAGACGATGACGCAGTTCCTAGAGCAGGGAGAGGCCACCCTCTCAGTGGCAAGAAGAGCCCTGGCTGCCCAGTTGCCAGTCCTACCACGGTCGGAGGTAACCTTCCTGGCTCCAGTCACACGACCAGATAAGGTGGTGTGTGTGGGCATGAATTATGTGGACCACTGCAAAGAACAGAACGTGCCCGTGCCCAAGGAGCCCATCATCTTCAGCAAGTTTGCCAGCTCCATCGTGGGGCCCTATGATGAGGTGGTCCTCCCACCACAGAGCCAGGAGGTAGATTGGGAAGTGGAGCTGGCCGTGGTCATTGGAAAGAAAGGCAAGCACATCAAGGCCACAGATGCTATGGCCCACGTGGCCGGCTTCACTGTGGCTCATGACGTGAGTGCTCGTGACTGGCAAATGAGACGTAATGGGAAACAATGGCTGCTGGGAAAAACCTTCGACACCTTCTGCCCTCTGGGCCCTGCCTTGGTGACCAAGGACAGTGTAGCAGATCCACACAACTTAAAGATCTGCTGCCGAGTGAATGGGGAAGTGGTCCAGAGCGGCAACACCAACCAGATGGTATTCAAGACAGAGGACCTGATAGCCTGGGTCTCCCAGTTTGTTACCTTTTACCCAGGGGATGTCATCCTAACTGGGACCCCCCCAGGTGTCGGTGTATTCAGGAAACCTCCTGTCTTTCTCAAGAAGGGGGATGAAGTCCAGTGTGAGATTGAAGAACTAGGTGTCATCATCAACAAGGTGGTGTGC

Insert Property: Insert 1

Type Value Extra Information
End on reference sequence 1098
Start on reference sequence 154

Inferred Whole Plasmid Sequence: (BETA)
Insert green, Vector black

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGATGCTGGTGTCTGGTAGAAGAAGGTTACTCACAGTTCTGCTGCAGGCTCAGAAGTGGCCCTTTCAACCCTCCAGAGACATGAGACTAGTGCAGTTCCGGGCACCCCACCTGGTGGGGCCTCACTTGGGCCTGGAGACAGGGAATGGTGGAGGGGTTATCAACCTCAATGCCTTTGACCCCACACTCCCGAAGACGATGACGCAGTTCCTAGAGCAGGGAGAGGCCACCCTCTCAGTGGCAAGAAGAGCCCTGGCTGCCCAGTTGCCAGTCCTACCACGGTCGGAGGTAACCTTCCTGGCTCCAGTCACACGACCAGATAAGGTGGTGTGTGTGGGCATGAATTATGTGGACCACTGCAAAGAACAGAACGTGCCCGTGCCCAAGGAGCCCATCATCTTCAGCAAGTTTGCCAGCTCCATCGTGGGGCCCTATGATGAGGTGGTCCTCCCACCACAGAGCCAGGAGGTAGATTGGGAAGTGGAGCTGGCCGTGGTCATTGGAAAGAAAGGCAAGCACATCAAGGCCACAGATGCTATGGCCCACGTGGCCGGCTTCACTGTGGCTCATGACGTGAGTGCTCGTGACTGGCAAATGAGACGTAATGGGAAACAATGGCTGCTGGGAAAAACCTTCGACACCTTCTGCCCTCTGGGCCCTGCCTTGGTGACCAAGGACAGTGTAGCAGATCCACACAACTTAAAGATCTGCTGCCGAGTGAATGGGGAAGTGGTCCAGAGCGGCAACACCAACCAGATGGTATTCAAGACAGAGGACCTGATAGCCTGGGTCTCCCAGTTTGTTACCTTTTACCCAGGGGATGTCATCCTAACTGGGACCCCCCCAGGTGTCGGTGTATTCAGGAAACCTCCTGTCTTTCTCAAGAAGGGGGATGAAGTCCAGTGTGAGATTGAAGAACTAGGTGTCATCATCAACAAGGTGGTGTGCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Information:

Vector Name:pENTR223
Type:bacterial plasmid
Description:Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Properties:recombinational cloning
Comments:Additional information about this vector is available from the commercial website and at the ORFeome website http://www.orfeomecollaboration.org/html/vectors.shtml; also see supplemental figure 1 of the paper PubMed ID 15489335.
Map:pDONR223.1.gif
Sequence:pENTR223-seq.doc
Size (bp):2815
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha T1 phage resistant Y

Antibiotic Selections:

Host Type Marker
bacterial spectinomycin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL spectinomycin Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Authors:

Author Name Author Type
The ORFeome Collaboration Consortium