Clone ID:	EvNO00285801
Is Verified:	N
Status:	AVAILABLE
Source:
Description:	Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.
Comments:	Empty vector contains the ccdB death cassette.
Type:	No insert
Verification Method:
Distribution:	Academic and non-profit labs
Special MTA:

Map:	pBY011_map.pdf

Related Identifiers:

Sequencing Information:

Phred Score:	896
Date:	2016-08-11

Last Passing Sequencing Read:

NGGGCACACAAAANNNGCCAAAAGAACGCTAGACATGATACGCCAGCTTG CATGCCATCACCACTTTGTACAAGAAAGCTGAACGAGAAACGTAAAATGA TATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACAT AATACTGTAAAACACAACATATCCAGTCACTATGGTCGACCTGCAGACTG GCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAA TGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCC CCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCA GCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTAT CTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGC GTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTC TCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGCCCCTGTTCTCG TCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCC TTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCAT TCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCT TGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTC ATAGCATACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATT CTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTT TAGTAAGCCGGATCCACGCGTTTACGCCCCGCCCTGCCACTCATCGCAGT ACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCCATCACAAAC GCATGATGAACCTGAATCGNCAGCGGCATCAGCACCTTGTCGCCTTGCGT ATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATAATGG CCCACGTTTAATCAAAACTGGTGAAACTCNACCCAGGATTGGCTGAGACG AAAACATATTCTCAATAAACCCTTTAGGGAAATAGNCCAGNTTTCACCCG TACACGCACATCTTGCGAATAATGTGTAGAACTGCCGGAATCGTCNGGGN ATCCTTCCGACCGATGAAACGTTCAGTTGCTCATGAAAAGGTGTTACAAG GGGAACCTTTCCATATTCCAATTCACGTCTTTCATTCCTACGGAATCCGG AGAACTTCTCAAGCGGCCAAAAGTGGAAAAGCCGGAAAAACTTGGCTTTT TTCTCCGGCCTTAAAGCCCAATTCCCCTAACGCCGGTTAGCCTTTAAACC

Vector Information:

Vector Name:	pBY011
Type:	bacterial plasmid
Description:	Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.
Properties:	Gateway, acceptor (destination), inducible expression, yeast expression, yeast shuttle
Comments:	This is a bacteria-yeast shuttle vector useful for expressing cDNAs in yeast that includes an auxotrophic marker for selection in yeast.
Map:	pBY011_map.pdf
Sequence:	pBY011.txt
Size (bp):	7920
Form:	dsDNA

Host Information:

Host Strain	Is Used In Distribution	Description
DB3.1 T1 phage resistant	Y

Antibiotic Selections:

Host Type	Marker
bacterial	ampicillin
bacterial	chloramphenicol

Recommended Growth Condition:

Host Type	Selection Condition	Growth Condition	Comments
bacterial	chloramphenicol, ampicillin	LB with 35ug/ml chloramphenicol and 100ug/ml ampicillin