Clone: EvNO00285801 ← Click Here To Order

Clone ID:EvNO00285801
Is Verified:N
Status:AVAILABLE
Source:
Description:Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.
Comments:Empty vector contains the ccdB death cassette.
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Sequencing Information:

Phred Score: 896
Date: 2016-08-11

Last Passing Sequencing Read:

NGGGCACACAAAANNNGCCAAAAGAACGCTAGACATGATACGCCAGCTTG CATGCCATCACCACTTTGTACAAGAAAGCTGAACGAGAAACGTAAAATGA TATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACAT AATACTGTAAAACACAACATATCCAGTCACTATGGTCGACCTGCAGACTG GCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAA TGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCC CCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCA GCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTAT CTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGC GTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTC TCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGCCCCTGTTCTCG TCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCC TTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCAT TCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCT TGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTC ATAGCATACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATT CTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTT TAGTAAGCCGGATCCACGCGTTTACGCCCCGCCCTGCCACTCATCGCAGT ACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCCATCACAAAC GCATGATGAACCTGAATCGNCAGCGGCATCAGCACCTTGTCGCCTTGCGT ATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATAATGG CCCACGTTTAATCAAAACTGGTGAAACTCNACCCAGGATTGGCTGAGACG AAAACATATTCTCAATAAACCCTTTAGGGAAATAGNCCAGNTTTCACCCG TACACGCACATCTTGCGAATAATGTGTAGAACTGCCGGAATCGTCNGGGN ATCCTTCCGACCGATGAAACGTTCAGTTGCTCATGAAAAGGTGTTACAAG GGGAACCTTTCCATATTCCAATTCACGTCTTTCATTCCTACGGAATCCGG AGAACTTCTCAAGCGGCCAAAAGTGGAAAAGCCGGAAAAACTTGGCTTTT TTCTCCGGCCTTAAAGCCCAATTCCCCTAACGCCGGTTAGCCTTTAAACC

Vector Information:

Vector Name:pBY011
Type:bacterial plasmid
Description:Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.
Properties:Gateway, acceptor (destination), inducible expression, yeast expression, yeast shuttle
Comments:This is a bacteria-yeast shuttle vector useful for expressing cDNAs in yeast that includes an auxotrophic marker for selection in yeast.
Map:pBY011_map.pdf
Sequence:pBY011.txt
Size (bp):7920
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DB3.1 T1 phage resistant Y

Antibiotic Selections:

Host Type Marker
bacterial ampicillin
bacterial chloramphenicol

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial chloramphenicol, ampicillin LB with 35ug/ml chloramphenicol and 100ug/ml ampicillin