Clone: EvNO00025966 ← Click Here To Order
| Clone ID: | EvNO00025966 |
| Is Verified: | U |
| Status: | AVAILABLE |
| Source: | J. Kamil in Coen lab (BCMP/HMS) |
| Description: | Shuttle vector for BAC mutagenesis (for example of infectious viral genome into BAC), ts for DNA replication; kanamycin resistance; in vivo selection for cointegrants followed by resolution to clone in BACs |
| Comments: | ADD THE PROTOCOL HERE !!! |
| Type: | No insert |
| Verification Method: | |
| Distribution: | Academic and non-profit labs |
| Special MTA: | |
Related Identifiers:
| Original Clone ID |
pST76K-SR |
| SYNONYM |
pST76K_SR |
Vector Information:
| Vector Name: | pST76K-SR |
| Type: | bacterial plasmid |
| Description: | Shuttle vector for RecA-mediated BAC mutagenesis, ts for DNA replication; kanamycin resistance; in vivo selection for cointegrants followed by resolution to manipulate BACs. |
| Properties: | BAC mutagenesis, RecA mutagenesis, multiple cloning site, mutagenesis vector |
| Comments: | Donor of the 'empty vector' clone of this vector provided a protocol for use; see clone description |
| Map: | pST76K-SR.pdf |
| Sequence: | pST76K-SR.txt |
| Size (bp): | 6326 |
| Form: | dsDNA |
Host Information:
| Host Strain |
Is Used In Distribution |
Description |
| EB5-alpha T1, T5 phage resistant |
Y |
EB5-alpha host as supplied by Edge BioSystems |
Antibiotic Selections:
| Host Type |
Marker |
| bacterial |
kanamycin |
Recommended Growth Condition:
| Host Type |
Selection Condition |
Growth Condition |
Comments |
| bacterial |
50 ug/mL kanamycin |
Useful for ts bacterial origin of replication clone (allows selection for integration into BACs at 41 degrees) |
Authors:
| Author Name |
Author Type |
| Donald Coen |
Adademic researcher, clone donor's PI |
| Jeremy Kamil |
Academic researcher, clone donor |
Publications:
| PMID |
Title |
| 15890938 |
vLIP, a viral lipase homologue, is a virulence factor of Marek's disease virus |
| 10933677 |
Fast screening procedures for random transposon libraries of cloned herpesvirus genomes: mutational analysis of human cytomegalovirus envelope glycoprotein genes |
| 10888635 |
Cloning and mutagenesis of the murine gammaherpesvirus 68 genome as an infectious bacterial artificial chromosome |
