Clone: EvNO00025966 ← Click Here To Order

Clone ID:EvNO00025966
Is Verified:U
Status:AVAILABLE
Source:J. Kamil in Coen lab (BCMP/HMS)
Description:Shuttle vector for BAC mutagenesis (for example of infectious viral genome into BAC), ts for DNA replication; kanamycin resistance; in vivo selection for cointegrants followed by resolution to clone in BACs
Comments:ADD THE PROTOCOL HERE !!!
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Original Clone ID pST76K-SR
SYNONYM pST76K_SR

Vector Information:

Vector Name:pST76K-SR
Type:bacterial plasmid
Description:Shuttle vector for RecA-mediated BAC mutagenesis, ts for DNA replication; kanamycin resistance; in vivo selection for cointegrants followed by resolution to manipulate BACs.
Properties:BAC mutagenesis, RecA mutagenesis, multiple cloning site, mutagenesis vector
Comments:Donor of the 'empty vector' clone of this vector provided a protocol for use; see clone description
Map:pST76K-SR.pdf
Sequence:pST76K-SR.txt
Size (bp):6326
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
EB5-alpha T1, T5 phage resistant Y EB5-alpha host as supplied by Edge BioSystems

Antibiotic Selections:

Host Type Marker
bacterial kanamycin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 50 ug/mL kanamycin Growth in LB/kanamycin (50 ug/mL) at 30 degrees C Useful for ts bacterial origin of replication clone (allows selection for integration into BACs at 41 degrees)

Authors:

Author Name Author Type
Donald Coen Adademic researcher, clone donor's PI
Jeremy Kamil Academic researcher, clone donor

Publications:

PMID Title
15890938 vLIP, a viral lipase homologue, is a virulence factor of Marek's disease virus
10933677 Fast screening procedures for random transposon libraries of cloned herpesvirus genomes: mutational analysis of human cytomegalovirus envelope glycoprotein genes
10888635 Cloning and mutagenesis of the murine gammaherpesvirus 68 genome as an infectious bacterial artificial chromosome