Related Identifiers:
Original Clone ID |
pST76K-SR |
SYNONYM |
pST76K_SR |
Vector Information:
Vector Name: | pST76K-SR |
Type: | bacterial plasmid |
Description: | Shuttle vector for RecA-mediated BAC mutagenesis, ts for DNA replication; kanamycin resistance; in vivo selection for cointegrants followed by resolution to manipulate BACs. |
Properties: | BAC mutagenesis, RecA mutagenesis, multiple cloning site, mutagenesis vector |
Comments: | Donor of the 'empty vector' clone of this vector provided a protocol for use; see clone description |
Map: | pST76K-SR.pdf |
Sequence: | pST76K-SR.txt |
Size (bp): | 6326 |
Form: | dsDNA |
Host Information:
Host Strain |
Is Used In Distribution |
Description |
EB5-alpha T1, T5 phage resistant |
Y |
EB5-alpha host as supplied by Edge BioSystems |
Antibiotic Selections:
Host Type |
Marker |
bacterial |
kanamycin |
Recommended Growth Condition:
Host Type |
Selection Condition |
Growth Condition |
Comments |
bacterial |
50 ug/mL kanamycin |
Useful for ts bacterial origin of replication clone (allows selection for integration into BACs at 41 degrees) |
Authors:
Author Name |
Author Type |
Donald Coen |
Adademic researcher, clone donor's PI |
Jeremy Kamil |
Academic researcher, clone donor |
Publications:
PMID |
Title |
15890938 |
vLIP, a viral lipase homologue, is a virulence factor of Marek's disease virus |
10933677 |
Fast screening procedures for random transposon libraries of cloned herpesvirus genomes: mutational analysis of human cytomegalovirus envelope glycoprotein genes |
10888635 |
Cloning and mutagenesis of the murine gammaherpesvirus 68 genome as an infectious bacterial artificial chromosome |