Clone: EvNO00025964

Clone ID:EvNO00025964
Is Verified:U
Source:J. Kamil in Coen lab (BCMP/HMS)
Description:Cloning and in vitro transcription vector with synthetic NTAP ORF added (synthetic NTAP designed to avoid repeats, optimize for human codon usage); amp resistance; restriction enzyme cloning.
Comments:Synthetic affinity tag ORF was designed in order to eliminate repeated elements present in the native CTAP described in PubMed 10504710
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Original Clone ID pSP72 syn-protein C-NTAP
GenBank Accession EU040031
GI 155197411

Vector Information:

Vector Name:pJK-NTAP
Type:bacterial plasmid
Description:Cloning vector with Protein C NTAP tag ORF (synthetic ORF eliminates nucleotide repeats present in wild-type); amp resistance; restriction enzyme cloning
Properties:cloning vector, in vitro transcription, multiple cloning site, with tag/fusion/marker
Comments:PTP N-terminal TAP tag (see PMID 16278461) added via synthetic construct that does not have direct DNA repeats present in original construct and is codon optimized for human
Size (bp):2930

Host Information:

Host Strain Is Used In Distribution Description
EB5-alpha T1, T5 phage resistant Y EB5-alpha host as supplied by Edge BioSystems

Antibiotic Selections:

Host Type Marker
bacterial ampicillin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.


Author Name Author Type
Donald Coen Adademic researcher, clone donor's PI
Jeremy Kamil Academic researcher, clone donor


PMID Title
16278461 Highly efficient tandem affinity purification of trypanosome protein complexes based on a novel epitope combination
17634236 Human cytomegalovirus protein kinase UL97 forms a complex with the tegument phosphoprotein pp65.