Clone: EvNO00025964 ← Click Here To Order

Clone ID:EvNO00025964
Is Verified:U
Status:AVAILABLE
Source:J. Kamil in Coen lab (BCMP/HMS)
Description:Cloning and in vitro transcription vector with synthetic NTAP ORF added (synthetic NTAP designed to avoid repeats, optimize for human codon usage); amp resistance; restriction enzyme cloning.
Comments:Synthetic affinity tag ORF was designed in order to eliminate repeated elements present in the native CTAP described in PubMed 10504710
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Original Clone ID pSP72 syn-protein C-NTAP
GenBank Accession EU040031
GI 155197411

Vector Information:

Vector Name:pJK-NTAP
Type:bacterial plasmid
Description:Cloning vector with Protein C NTAP tag ORF (synthetic ORF eliminates nucleotide repeats present in wild-type); amp resistance; restriction enzyme cloning
Properties:cloning vector, in vitro transcription, multiple cloning site, with tag/fusion/marker
Comments:PTP N-terminal TAP tag (see PMID 16278461) added via synthetic construct that does not have direct DNA repeats present in original construct and is codon optimized for human
Map:pSP72-syn-Protein_C-NTAP.pdf
Sequence:pSP72-syn-Protein_C-NTAP.txt
Size (bp):2930
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
EB5-alpha T1, T5 phage resistant Y EB5-alpha host as supplied by Edge BioSystems

Antibiotic Selections:

Host Type Marker
bacterial ampicillin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type
Donald Coen Adademic researcher, clone donor's PI
Jeremy Kamil Academic researcher, clone donor

Publications:

PMID Title
16278461 Highly efficient tandem affinity purification of trypanosome protein complexes based on a novel epitope combination
17634236 Human cytomegalovirus protein kinase UL97 forms a complex with the tegument phosphoprotein pp65.