Clone: EvNO00025963

Clone ID:EvNO00025963
Is Verified:U
Source:J. Kamil in Coen lab (BCMP/HMS)
Description:Vector with kanR gene inside CTAP tag ORF (synthetic CTAP designed to avoid repeats, optimize for human codon usage); useful for two-step Red recombination BAC mutagenesis.
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Original Clone ID pSP72 syn-CTAP AphAI

Vector Information:

Vector Name:pSP72 syn-CTAP AphAI
Type:bacterial plasmid
Description:Vector for BAC mutagenesis via Red recombination (kanR excised upon I-SceI and Rec E/T induction) with disrupted affinity tag (synthetic ORF eliminates nucleotide repeats present in wild-type form); kanamycin resistance; restriction enzyme cloning.
Properties:BAC mutagenesis, I-SceI site, Red recombination, in vitro transcription, multiple cloning site, mutagenesis vector, with tag/fusion/marker
Comments:See pSP72-syn-CTAP for map without the kanR (AphAI) insert and I-SceI site that make this useful for BAC mutagenesis
Size (bp):3968

Host Information:

Host Strain Is Used In Distribution Description
EB5-alpha T1, T5 phage resistant Y EB5-alpha host as supplied by Edge BioSystems

Antibiotic Selections:

Host Type Marker
bacterial kanamycin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 50ug/mL kanamycin Growth with the single antibiotic in LB at 37 degrees is recommended. Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.


Author Name Author Type
Donald Coen Adademic researcher, clone donor's PI
Jeremy Kamil Academic researcher, clone donor


PMID Title
16526409 Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli
10504710 A generic protein purification method for protein complex characterization and proteome exploration