Clone: EvNO00025963 ← Click Here To Order

Clone ID:EvNO00025963
Is Verified:U
Status:AVAILABLE
Source:J. Kamil in Coen lab (BCMP/HMS)
Description:Vector with kanR gene inside CTAP tag ORF (synthetic CTAP designed to avoid repeats, optimize for human codon usage); useful for two-step Red recombination BAC mutagenesis.
Comments:
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Original Clone ID pSP72 syn-CTAP AphAI
SYNONYM pSP72-syn-CTAP-AphAI

Vector Information:

Vector Name:pSP72 syn-CTAP AphAI
Type:bacterial plasmid
Description:Vector for BAC mutagenesis via Red recombination (kanR excised upon I-SceI and Rec E/T induction) with disrupted affinity tag (synthetic ORF eliminates nucleotide repeats present in wild-type form); kanamycin resistance; restriction enzyme cloning.
Properties:BAC mutagenesis, I-SceI site, Red recombination, in vitro transcription, multiple cloning site, mutagenesis vector, with tag/fusion/marker
Comments:See pSP72-syn-CTAP for map without the kanR (AphAI) insert and I-SceI site that make this useful for BAC mutagenesis
Map:pSP72-syn-CTAP-AphAI.pdf
Sequence:pSP72-syn-CTAP-AphAI.txt
Size (bp):3968
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
EB5-alpha T1, T5 phage resistant Y EB5-alpha host as supplied by Edge BioSystems

Antibiotic Selections:

Host Type Marker
bacterial kanamycin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 50ug/mL kanamycin Growth with the single antibiotic in LB at 37 degrees is recommended. Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Authors:

Author Name Author Type
Donald Coen Adademic researcher, clone donor's PI
Jeremy Kamil Academic researcher, clone donor

Publications:

PMID Title
16526409 Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli
10504710 A generic protein purification method for protein complex characterization and proteome exploration