Clone ID:	EvNO00025962
Is Verified:	U
Status:	AVAILABLE
Source:	J. Kamil in Coen lab (BCMP/HMS)
Description:	Cloning and in vitro transcription vector with synthetic CTAP ORF added; amp resistance; restriction enzyme cloning.
Comments:	Synthetic affinity tag ORF was designed in order to eliminate repeated elements present in the native CTAP described in PubMed 10504710
Type:	No insert
Verification Method:
Distribution:	Academic and non-profit labs
Special MTA:

Map:	pSP72synCTAP.pdf

Related Identifiers:

Original Clone ID	pSP72 syn-CTAP
SYNONYM	pSP72-syn-CTAP

Vector Information:

Vector Name:	pSP72 syn-CTAP
Type:	bacterial plasmid
Description:	Cloning vector with CTAP tag ORF (synthetic ORF eliminates nucleotide repeats present in wild-type form), SP6 and T7 promoters; amp resistance; restriction enzyme cloning.
Properties:	cloning vector, in vitro transcription, multiple cloning site, with tag/fusion/marker
Comments:	C-terminal TAP tag (see PMID 10504710) added via synthetic construct that does not have direct DNA repeats present in original construct and is codon optimized for human
Map:	pSP72synCTAP.pdf
Sequence:	pSP72synCTAP.txt
Size (bp):	3017
Form:	dsDNA

Host Information:

Host Strain	Is Used In Distribution	Description
EB5-alpha T1, T5 phage resistant	Y	EB5-alpha host as supplied by Edge BioSystems

Antibiotic Selections:

Host Type	Marker
bacterial	ampicillin

Recommended Growth Condition:

Host Type	Selection Condition	Growth Condition	Comments
bacterial	100 ug/mL ampicillin	Growth with the single antibiotic in LB at 37 degrees is recommended.	Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name	Author Type
Donald Coen	Adademic researcher, clone donor's PI
Jeremy Kamil	Academic researcher, clone donor

Publications:

PMID	Title
10504710	A generic protein purification method for protein complex characterization and proteome exploration