Related Identifiers:
Original Clone ID |
pSP72 syn-CTAP |
SYNONYM |
pSP72-syn-CTAP |
Vector Information:
Vector Name: | pSP72 syn-CTAP |
Type: | bacterial plasmid |
Description: | Cloning vector with CTAP tag ORF (synthetic ORF eliminates nucleotide repeats present in wild-type form), SP6 and T7 promoters; amp resistance; restriction enzyme cloning. |
Properties: | cloning vector, in vitro transcription, multiple cloning site, with tag/fusion/marker |
Comments: | C-terminal TAP tag (see PMID 10504710) added via synthetic construct that does not have direct DNA repeats present in original construct and is codon optimized for human |
Map: | pSP72synCTAP.pdf |
Sequence: | pSP72synCTAP.txt |
Size (bp): | 3017 |
Form: | dsDNA |
Host Information:
Host Strain |
Is Used In Distribution |
Description |
EB5-alpha T1, T5 phage resistant |
Y |
EB5-alpha host as supplied by Edge BioSystems |
Antibiotic Selections:
Host Type |
Marker |
bacterial |
ampicillin |
Recommended Growth Condition:
Host Type |
Selection Condition |
Growth Condition |
Comments |
bacterial |
100 ug/mL ampicillin |
Commonly used conditions for ampicillin resistant plasmid clones. |
Authors:
Author Name |
Author Type |
Donald Coen |
Adademic researcher, clone donor's PI |
Jeremy Kamil |
Academic researcher, clone donor |
Publications:
PMID |
Title |
10504710 |
A generic protein purification method for protein complex characterization and proteome exploration |