Clone: EvNO00025951

Clone ID:EvNO00025951
Is Verified:U
Status:AVAILABLE
Source:J. Kamil in Coen lab (BCMP/HMS)
Description:Transposable baculovirus expression vector for use with Invitrogen Bac-to-Bac system, has polyhedrin promoter, encodes N-terminal GST and Prescission Protease site prior to MCS; amp resistance (gentamicin for recombinant BACmid selection after transposition); standard restriction enyzme or CpoI-based in-frame single-cut directional cloning.
Comments: Has failed multiple sequencing reads using M13R as well as AMP primers.
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Original Clone ID pFASTBAC1 GST PP Cpo

Vector Information:

Vector Name:pFASTBAC1 GST PP Cpo
Type:bacterial plasmid
Description:Transposable baculovirus expression vector for use with Invitrogen Bac-to-Bac system, has polyhedrin promoter, encodes N-terminal GST and Prescission Protease site prior to MCS; amp resistance (gentamicin for recombinant BACmid selection after transposition); standard restriction enyzme or CpoI-based in-frame single-cut directional cloning.
Properties:CpoI enzyme-based directional cloning, baculovirus/insect cell expression, baculovirus/insect cell transposition, multiple cloning site, with tag/fusion/marker
Comments:
Map:pFASTBAC1-GST-PP-Cpo.pdf
Sequence:pFASTBAC1-GST-PP-Cpo.txt
Size (bp):5462
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
EB5-alpha T1, T5 phage resistant Y EB5-alpha host as supplied by Edge BioSystems

Antibiotic Selections:

Host Type Marker
bacterial ampicillin
insect cells gentamicin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type
Donald Coen Adademic researcher, clone donor's PI
Jeremy Kamil Academic researcher, clone donor

Publications:

PMID Title
8392598 Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli