Clone: EvNO00025949

Clone ID:EvNO00025949
Is Verified:U
Status:AVAILABLE
Source:O'Shea lab (HU/HMS)
Description:Yeast fluorescent (RFP) vector with PTEF promoter; amp resistance (kanamycin for yeast); restriction enzyme cloning.
Comments:Similar but not identical to pKT146, pKT176, pKT178 clones also in the repository collection.
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:TSIEN

Related Identifiers:

Original Clone ID pFA6a-mRFP1-kanMX6
SYNONYM pFA6a-RFP-kanR

Sequencing Information:

Phred Score: 863
Date: 2019-08-19

Last Passing Sequencing Read:

NNNNCCACGAATCCGCGCTAGCCATAGTGGATCTGATATCATCGATGAAT TCGAGCTCGTTTAAACTGGATGGCGGCGTTAGTATCGAATCGACAGCAGT ATAGCGACCAGCATTCACATACGATTGACGCATGATATTACTTTCTGCGC ACTTAACTTCGCATCTGGGCAGATGATGTCGAGGCGAAAAAAAATATAAA TCACGCTAACATTTGATTAAAATAGAACAACTACAATATAAAAAAACTAT ACAAATGACAAGTTCTTGAAAACAAGAATCTTTTTATTGTCAGTACTGAT TAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGG ATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAA ACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCG ATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAA AATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTG AGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAG CCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCAT TCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGAC AATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCA TCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGC TGTTTTGCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTAC GGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTT AGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATG TTTCAGAAACAACTCTGGCGCATCGGGCTTTCCATANCATCGATAGATTG TCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTNATACCATATAAA TCAGCATCCATGTTGGAATTTNNATCGCGCCTCGAAACGTGAGTCTTTTC CTTACCATGGNNTGTTATGTTCGGATGTGATGTGAGAACTGTATCCTAGN CAGATTTTTAAAGGAGTAATGAANGAAGAACTTCGTGGGCAATCCTACCN TTTTAAATTCTCTACGGGNNCCCGCGTGGGAACAATCACGCGGTCGTGAG GGGACGTTCCCTGCTCCCAGCTTCCCAAGAACCCAAATTTTGCTCCGCCC GGGCCCTCCAAGTGTGGATGCAAGAATTAACTGGGATTATGCCAAAGTTA GGGAATACATTTGCCCGAGTCCCCTAAAATTTAAAAGGATTGGCCCCTTT

Vector Information:

Vector Name:pFA6a-mRFP1-kanMX6
Type:bacterial plasmid
Description:Yeast fluorescent (RFP) vector with PTEF promoter; amp resistance (kanamycin for yeast); restriction enzyme cloning.
Properties:fluorescent marker, multiple cloning site, yeast expression, yeast shuttle
Comments:
Map:pFA6a-mRFP-KanMX6.pdf
Sequence:pFA6a-mRFP-KanMX6.txt
Size (bp):4846
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha T1 phage resistant Y

Antibiotic Selections:

Host Type Marker
bacterial ampicillin
yeast kanamycin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type
Erin O'Shea Academic reseracher, clone PI
James Falvo Academic researcher, clone donor and author
WK Huh Academic researcher, clone first author

Publications:

PMID Title
14562095 Global analysis of protein localization in budding yeast