Clone: EvNO00025939
| Clone ID: | EvNO00025939 |
| Is Verified: | U |
| Status: | AVAILABLE |
| Source: | J. Kamil in Coen lab (BCMP/HMS) |
| Description: | Mammalian and in vitro expression vector with EF1-alpha promoter and T7, adds C-terminal V5 epitope then His tag; amp resistance (neomycin for mammalian selection); CpoI-based in-frame, single-cut directional cloning. |
| Comments: | |
| Type: | No insert |
| Verification Method: | |
| Distribution: | Academic and non-profit labs |
| Special MTA: |
Related Identifiers:
| Original Clone ID | pEF1 Cpo V5 His |
Sequencing Information:
| Phred Score: | 809 |
| Date: | 2014-10-20 |
Last Passing Sequencing Read:
CNCGGCAGTACTGGTACGGTCCGAGTCACCCATTCGAAGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACG CGTACCGGTCATCATCACCATCACCATTGAGTTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATC TGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAA TTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAA GACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTA TCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCG CCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGG GGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAG TGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAA CTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAAGGNATTTTGCCGATTTCGGCCTATTGGTTAAA AAATGAGCTGATTTAACAAAAATTTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTANGGTGTGGAAAAGTCCCCA GGCTCCCCAGCAGGCAGAANTATGCAANCATGCATCTCAATTANTCAGCACCAGGTGTGGAAAGTCCCCAGGCTCCCNAC CAGGCAAAATATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAANTCCCCCCCTAAATCCGNCCNATTCCGNCCCTA AATTCNGNCCANTTTCNCCCAATTTCCCCCCCNANGGNTGAAAAAATTTTTTTTATTTTATGCAAANGGCCAANGNCCCC CCNNGCCCTTTNAAATNTTNCNAAAAAAATGGANGAAGNTTTTTTTGGANACCNAAANNTTTTNAAAAAAANCNCCNGGA ANNTTTTAAACCNTTTTNGGANTTNAAAAAAAAAAAGAAAAAAANNTTTTCCCAAANTNAAAAAAAAGATTTCCCCNGTT TCCCCCCCTTTGGGGNANAANTTTNTNTAAANNGGAAAAAAAAAANNNTTTTTANAACCCNNTTTNTTTTTAAAAAAGGN CCNNTN
Vector Information:
| Vector Name: | pEF1 Cpo V5 His |
| Type: | bacterial plasmid |
| Description: | Mammalian and in vitro expression vector with EF1-alpha promoter and T7, adds C-eterminal V5 then His tag; amp resistance (neomycin for mammalian selection); CpoI-based in-frame, single-cut directional cloning. |
| Properties: | CpoI enzyme-based directional cloning, in vitro transcription, mammalian expression, multiple cloning site, ssDNA production, with tag/fusion/marker |
| Comments: | Cloning into CpoI site can be used to create in-frame C-terminal V5, His tagged fusion ORF |
| Map: | pEF1-Cpo-V5-His.pdf |
| Sequence: | pEF1-Cpo-V5-His.txt |
| Size (bp): | 6121 |
| Form: | dsDNA |
Host Information:
| Host Strain | Is Used In Distribution | Description |
| EB5-alpha T1, T5 phage resistant | Y | EB5-alpha host as supplied by Edge BioSystems |
Antibiotic Selections:
| Host Type | Marker |
| bacterial | ampicillin |
Recommended Growth Condition:
| Host Type | Selection Condition | Growth Condition | Comments |
| bacterial | 100 ug/mL ampicillin | Growth with the single antibiotic in LB at 37 degrees is recommended. | Commonly used conditions for ampicillin resistant plasmid clones. |
Authors:
| Author Name | Author Type |
| Donald Coen | Adademic researcher, clone donor's PI |
| Jeremy Kamil | Academic researcher, clone donor |
