Clone: EvNO00025939 ← Click Here To Order

Clone ID:EvNO00025939
Is Verified:U
Status:AVAILABLE
Source:J. Kamil in Coen lab (BCMP/HMS)
Description:Mammalian and in vitro expression vector with EF1-alpha promoter and T7, adds C-terminal V5 epitope then His tag; amp resistance (neomycin for mammalian selection); CpoI-based in-frame, single-cut directional cloning.
Comments:
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:

Related Identifiers:

Original Clone ID pEF1 Cpo V5 His

Sequencing Information:

Phred Score: 809
Date: 2014-10-20

Last Passing Sequencing Read:

CNCGGCAGTACTGGTACGGTCCGAGTCACCCATTCGAAGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACG CGTACCGGTCATCATCACCATCACCATTGAGTTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATC TGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAA TTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAA GACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTA TCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCG CCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGG GGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAG TGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAA CTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAAGGNATTTTGCCGATTTCGGCCTATTGGTTAAA AAATGAGCTGATTTAACAAAAATTTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTANGGTGTGGAAAAGTCCCCA GGCTCCCCAGCAGGCAGAANTATGCAANCATGCATCTCAATTANTCAGCACCAGGTGTGGAAAGTCCCCAGGCTCCCNAC CAGGCAAAATATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAANTCCCCCCCTAAATCCGNCCNATTCCGNCCCTA AATTCNGNCCANTTTCNCCCAATTTCCCCCCCNANGGNTGAAAAAATTTTTTTTATTTTATGCAAANGGCCAANGNCCCC CCNNGCCCTTTNAAATNTTNCNAAAAAAATGGANGAAGNTTTTTTTGGANACCNAAANNTTTTNAAAAAAANCNCCNGGA ANNTTTTAAACCNTTTTNGGANTTNAAAAAAAAAAAGAAAAAAANNTTTTCCCAAANTNAAAAAAAAGATTTCCCCNGTT TCCCCCCCTTTGGGGNANAANTTTNTNTAAANNGGAAAAAAAAAANNNTTTTTANAACCCNNTTTNTTTTTAAAAAAGGN CCNNTN

Vector Information:

Vector Name:pEF1 Cpo V5 His
Type:bacterial plasmid
Description:Mammalian and in vitro expression vector with EF1-alpha promoter and T7, adds C-eterminal V5 then His tag; amp resistance (neomycin for mammalian selection); CpoI-based in-frame, single-cut directional cloning.
Properties:CpoI enzyme-based directional cloning, in vitro transcription, mammalian expression, multiple cloning site, ssDNA production, with tag/fusion/marker
Comments:Cloning into CpoI site can be used to create in-frame C-terminal V5, His tagged fusion ORF
Map:pEF1-Cpo-V5-His.pdf
Sequence:pEF1-Cpo-V5-His.txt
Size (bp):6121
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
EB5-alpha T1, T5 phage resistant Y EB5-alpha host as supplied by Edge BioSystems

Antibiotic Selections:

Host Type Marker
bacterial ampicillin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type
Donald Coen Adademic researcher, clone donor's PI
Jeremy Kamil Academic researcher, clone donor