Clone: ScCD00023401

Clone ID:ScCD00023401
Is Verified:Y
Status:AVAILABLE
Source:HIP
Description:
Comments:
Type:cDNA
Verification Method:Sequence Verification
Distribution:No restriction
Special MTA:
Map:

Related Identifiers:

Original Clone ID FLH001719.01X
GenBank Accession AY723778
GI 51830242
HIP Clone ID 1719
HIP Master Clone ID 1277

Insert Information:

Insert Size (bp) Species Format Tissue Source Species Specific ID Gene Symbol Gene Name Keyword
1 2130 Saccharomyces cerevisiae CLOSED Genomic DNA template YDR146C SWI5 Transcription factor that activates transcription of genes expressed in G1 phase and at the G1/M boundary; localization to the nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase
Insert1
Size (bp)2130
SpeciesSaccharomyces cerevisiae
FormatCLOSED
Tissue SourceGenomic DNA template
Species Specific ID YDR146C
Gene SymbolSWI5
Gene NameTranscription factor that activates transcription of genes expressed in G1 phase and at the G1/M boundary; localization to the nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase
Keyword

Insert Sequence:

ATGGATACATCAAACTCTTGGTTTGATGCCTCAAAAGTACAAAGCCTAAATTTTGACCTACAAACCAACTCTTATTACTCAAATGCTAGAGGCAGTGACCCTTCGAGTTATGCAATAGAAGGCGAATATAAAACACTAGCAACAGATGATTTAGGCAATATATTGAACCTAAACTATGGTGAAACCAACGAAGTTATAATGAATGAAATAAATGACTTAAACCTTCCGCTGGGACCACTTTCTGATGAAAAATCTGTCAAGGTTTCTACTTTCTCCGAGTTAATAGGAAATGATTGGCAAAGTATGAACTTTGACTTAGAAAATAATTCAAGAGAAGTGACTCTAAATGCAACTAGCTTGTTGAATGAAAATAGATTAAATCAAGACAGTGGTATGACTGTTTATCAAAAAACAATGAGTGATAAGCCCCACGATGAAAAGAAGATTTCCATGGCGGATAATTTATTGTCGACTATAAACAAAAGTGAAATTAATAAAGGCTTCGATAGAAACCTCGGGGAATTACTGTTACAGCAACAACAAGAGTTGCGCGAGCAATTGAGAGCACAACAAGAGGCTAATAAGAAGTTAGAGTTAGAACTCAAACAAACACAATACAAGCAACAACAACTACAGGCTACCTTAGAGAACTCTGATGGTCCACAGTTTTTATCTCCCAAAAGGAAGATCTCTCCTGCTTCAGAAAATGTGGAGGATGTATACGCAAATAGCCTTTCACCAATGATTTCCCCACCAATGTCTAATACTTCGTTCACTGGGTCACCTTCTAGGAGAAACAATAGACAAAAGTACTGCCTGCAGAGAAAAAACTCTAGTGGTACTGTAGGACCACTATGTTTCCAGGAATTGAACGAAGGTTTCAATGACTCCCTCATTTCACCAAAGAAAATTCGCTCGAATCCAAATGAAAACCTCAGTTCAAAAACCAAATTTATTACGCCCTTCACTCCAAAGAGTAGAGTTAGTTCAGCTACGTCCAACTCTGCTAACATTACCCCTAATAACTTAAGGTTAGATTTCAAGATTAATGTTGAAGATCAGGAAAGCGAATATTCAGAGAAACCTTTGGGCCTGGGTATTGAACTTCTTGGAAAACCAGGTCCTTCTCCTACAAAATCAGTGTCTTTAAAGAGTGCTTCTGTGGATATTATGCCTACAATTCCTGGGTCTGTAAATAATACTCCATCAGTCAATAAAGTATCCCTTTCCTCCAGTTATATAGACCAATATACACCAAGAGGTAAGCAGTTACACTTTAGCTCTATCAGTGAGAATGCATTGGGTATCAATGCTGCGACACCACATCTAAAGCCGCCGAGCCAGCAAGCACGACATCGGGAAGGCGTTTTCAATGATTTAGACCCTAATGTACTTACGAAAAATACAGACAATGAAGGAGATGATAATGAGGAAAATGAGCCTGAAAGTAGATTTGTTATTTCCGAAACGCCTTCTCCCGTTCTTAAGTCACAAAGTAAGTACGAAGGAAGATCTCCTCAATTCGGCACACACATTAAGAAAATCAACACATATACCACAAATAGTCCCTCAAAAATCACAAGAAAACTCACAACACTGCCCAGAGGTTCAATCGATAAATATGTGAAGGAAATGCCTGATAAAACATTTGAATGTTTATTTCCTGGATGTACAAAAACATTCAAGAGAAGGTATAACATCAGATCTCATATTCAAACACATTTGGAAGATCGGCCATATTCCTGTGATCACCCTGGATGTGATAAGGCATTTGTACGAAATCATGACTTGATAAGACACAAAAAATCGCACCAAGAAAAGGCGTATGCATGTCCCTGTGGTAAGAAATTCAATAGAGAAGACGCTTTGGTTGTGCACAGAAGTAGAATGATTTGCAGCGGTGGTAAAAAGTATGAAAACGTGGTAATTAAAAGGTCTCCAAGAAAAAGAGGAAGACCAAGAAAAGATGGAACTTCAAGTGTTTCTAGTAGTCCAATCAAAGAAAATATTAACAAGGATCATAATGGACAGTTGATGTTCAAGCTTGAAGATCAACTCAGAAGAGAGCGTAGTTATGATGGGAATGGAACGGGGATTATGGTTTCGCCAATGAAAACTAATCAAAGGTAA

Insert Property: Insert 1

Type Value Extra Information
Discrepancy g1540a,E514K
End on reference sequence 2130
Start on reference sequence 1

Inferred Whole Plasmid Sequence: (BETA)
Insert green, Vector black

AGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCACCATGGATACATCAAACTCTTGGTTTGATGCCTCAAAAGTACAAAGCCTAAATTTTGACCTACAAACCAACTCTTATTACTCAAATGCTAGAGGCAGTGACCCTTCGAGTTATGCAATAGAAGGCGAATATAAAACACTAGCAACAGATGATTTAGGCAATATATTGAACCTAAACTATGGTGAAACCAACGAAGTTATAATGAATGAAATAAATGACTTAAACCTTCCGCTGGGACCACTTTCTGATGAAAAATCTGTCAAGGTTTCTACTTTCTCCGAGTTAATAGGAAATGATTGGCAAAGTATGAACTTTGACTTAGAAAATAATTCAAGAGAAGTGACTCTAAATGCAACTAGCTTGTTGAATGAAAATAGATTAAATCAAGACAGTGGTATGACTGTTTATCAAAAAACAATGAGTGATAAGCCCCACGATGAAAAGAAGATTTCCATGGCGGATAATTTATTGTCGACTATAAACAAAAGTGAAATTAATAAAGGCTTCGATAGAAACCTCGGGGAATTACTGTTACAGCAACAACAAGAGTTGCGCGAGCAATTGAGAGCACAACAAGAGGCTAATAAGAAGTTAGAGTTAGAACTCAAACAAACACAATACAAGCAACAACAACTACAGGCTACCTTAGAGAACTCTGATGGTCCACAGTTTTTATCTCCCAAAAGGAAGATCTCTCCTGCTTCAGAAAATGTGGAGGATGTATACGCAAATAGCCTTTCACCAATGATTTCCCCACCAATGTCTAATACTTCGTTCACTGGGTCACCTTCTAGGAGAAACAATAGACAAAAGTACTGCCTGCAGAGAAAAAACTCTAGTGGTACTGTAGGACCACTATGTTTCCAGGAATTGAACGAAGGTTTCAATGACTCCCTCATTTCACCAAAGAAAATTCGCTCGAATCCAAATGAAAACCTCAGTTCAAAAACCAAATTTATTACGCCCTTCACTCCAAAGAGTAGAGTTAGTTCAGCTACGTCCAACTCTGCTAACATTACCCCTAATAACTTAAGGTTAGATTTCAAGATTAATGTTGAAGATCAGGAAAGCGAATATTCAGAGAAACCTTTGGGCCTGGGTATTGAACTTCTTGGAAAACCAGGTCCTTCTCCTACAAAATCAGTGTCTTTAAAGAGTGCTTCTGTGGATATTATGCCTACAATTCCTGGGTCTGTAAATAATACTCCATCAGTCAATAAAGTATCCCTTTCCTCCAGTTATATAGACCAATATACACCAAGAGGTAAGCAGTTACACTTTAGCTCTATCAGTGAGAATGCATTGGGTATCAATGCTGCGACACCACATCTAAAGCCGCCGAGCCAGCAAGCACGACATCGGGAAGGCGTTTTCAATGATTTAGACCCTAATGTACTTACGAAAAATACAGACAATGAAGGAGATGATAATGAGGAAAATGAGCCTGAAAGTAGATTTGTTATTTCCGAAACGCCTTCTCCCGTTCTTAAGTCACAAAGTAAGTACGAAGGAAGATCTCCTCAATTCGGCACACACATTAAGAAAATCAACACATATACCACAAATAGTCCCTCAAAAATCACAAGAAAACTCACAACACTGCCCAGAGGTTCAATCGATAAATATGTGAAGGAAATGCCTGATAAAACATTTGAATGTTTATTTCCTGGATGTACAAAAACATTCAAGAGAAGGTATAACATCAGATCTCATATTCAAACACATTTGGAAGATCGGCCATATTCCTGTGATCACCCTGGATGTGATAAGGCATTTGTACGAAATCATGACTTGATAAGACACAAAAAATCGCACCAAGAAAAGGCGTATGCATGTCCCTGTGGTAAGAAATTCAATAGAGAAGACGCTTTGGTTGTGCACAGAAGTAGAATGATTTGCAGCGGTGGTAAAAAGTATGAAAACGTGGTAATTAAAAGGTCTCCAAGAAAAAGAGGAAGACCAAGAAAAGATGGAACTTCAAGTGTTTCTAGTAGTCCAATCAAAGAAAATATTAACAAGGATCATAATGGACAGTTGATGTTCAAGCTTGAAGATCAACTCAGAAGAGAGCGTAGTTATGATGGGAATGGAACGGGGATTATGGTTTCGCCAATGAAAACTAATCAAAGGTAAGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAA

Vector Information:

Vector Name:pDONR201
Type:bacterial plasmid
Description:Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Properties:Gateway, donor (entry), recombinational cloning
Comments:The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Map:pdonr201_pdonr207_map.pdf
Sequence:pDONR201_FASTA.txt
Size (bp):4470
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha T1 phage resistant Y

Antibiotic Selections:

Host Type Marker
bacterial kanamycin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 50ug/mL kanamycin Growth with the single antibiotic in LB at 37 degrees is recommended. Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Authors:

Author Name Author Type
HIP Academic Institute

Publications:

PMID Title
17322287 Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.