Clone: ScCD00023359

Clone ID:ScCD00023359
Is Verified:Y
Status:AVAILABLE
Source:HIP
Description:
Comments:
Type:cDNA
Verification Method:Sequence Verification
Distribution:No restriction
Special MTA:
Map:

Related Identifiers:

HIP Clone ID 1003
Original Clone ID FLH001003.01X
HIP Master Clone ID 561
GenBank Accession AY692770
GI 51012990

Insert Information:

Insert Size (bp) Species Format Tissue Source Species Specific ID Gene Symbol Gene Name Keyword
1 1311 Saccharomyces cerevisiae CLOSED Genomic DNA template YBR236C ABD1 Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA
Insert1
Size (bp)1311
SpeciesSaccharomyces cerevisiae
FormatCLOSED
Tissue SourceGenomic DNA template
Species Specific ID YBR236C
Gene SymbolABD1
Gene NameMethyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA
Keyword

Insert Sequence:

ATGTCAACCAAACCAGAGAAGCCAATATGGATGTCACAAGAAGACTATGACCGTCAGTACGGCTCAATTACTGGTGATGAATCGTCCACAGTCTCAAAAAAAGACTCCAAAGTTACTGCCAATGCGCCTGGTGATGGTAATGGATCATTACCAGTTTTGCAAAGCAGCTCTATCCTTACTTCTAAGGTGTCTGATCTACCCATTGAGGCAGAATCCGGGTTTAAAATCCAAAAGAGAAGACATGAAAGGTATGATCAAGAGGAAAGATTACGCAAACAGCGTGCTCAAAAATTGAGAGAAGAGCAACTAAAGAGACATGAAATAGAGATGACTGCAAATAGATCCATAAATGTTGACCAGATTGTCCGAGAGCACTGTAATGAACGTACAATAATCGCCAATCGCGCTAAGAGGAATCTATCGCCTATTATTAAGCTTCGTAATTTTAACAATGCGATTAAGTATATGTTAATTGACAAATATACAAAACCTGGAGATGTCGTTTTAGAACTCGGATGTGGCAAAGGTGGTGATTTAAGAAAATATGGTGCTGCAGGTATTTCACAGTTTATTGGTATTGACATTTCCAATGCCTCTATCCAAGAAGCTCATAAGAGATACCGGTCCATGAGAAATTTGGACTACCAAGTTGTGCTAATTACTGGGGATTGCTTTGGCGAATCATTAGGTGTTGCAGTAGAGCCCTTCCCAGACTGTAGATTTCCGTGTGATATCGTTTCGACGCAATTTTGCTTGCATTATGCTTTTGAGACCGAAGAAAAAGCAAGGAGAGCTTTACTGAATGTCGCCAAATCACTCAAAATTGGTGGTCACTTTTTTGGCACGATACCAGATTCAGAATTCATTCGTTATAAACTGAATAAATTTCCCAAAGAAGTGGAAAAGCCATCTTGGGGAAACTCCATTTATAAAGTGACATTTGAGAATAATTCATACCAAAAGAATGATTATGAGTTCACATCGCCTTATGGTCAAATGTATACTTATTGGCTAGAAGATGCTATAGATAATGTTCCTGAATATGTTGTGCCATTCGAAACCTTAAGAAGTTTGGCTGATGAATACGGTTTGGAACTAGTATCGCAAATGCCTTTTAATAAATTTTTTGTCCAAGAAATTCCCAAATGGATTGAGAGGTTTTCGCCTAAAATGAGGGAAGGTTTACAAAGATCTGATGGTAGATATGGTGTTGAAGGGGATGAAAAGGAAGCGGCATCATATTTCTATACCATGTTTGCATTCCGTAAGGTAAAACAGTATATCGAACCGGAAAGCGTAAAGCCCAACTGA

Insert Property: Insert 1

Type Value Extra Information
Discrepancy a377g,Y126C
End on reference sequence 1311
Start on reference sequence 1

Inferred Whole Plasmid Sequence: (BETA)
Insert green, Vector black

AGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCACCATGTCAACCAAACCAGAGAAGCCAATATGGATGTCACAAGAAGACTATGACCGTCAGTACGGCTCAATTACTGGTGATGAATCGTCCACAGTCTCAAAAAAAGACTCCAAAGTTACTGCCAATGCGCCTGGTGATGGTAATGGATCATTACCAGTTTTGCAAAGCAGCTCTATCCTTACTTCTAAGGTGTCTGATCTACCCATTGAGGCAGAATCCGGGTTTAAAATCCAAAAGAGAAGACATGAAAGGTATGATCAAGAGGAAAGATTACGCAAACAGCGTGCTCAAAAATTGAGAGAAGAGCAACTAAAGAGACATGAAATAGAGATGACTGCAAATAGATCCATAAATGTTGACCAGATTGTCCGAGAGCACTGTAATGAACGTACAATAATCGCCAATCGCGCTAAGAGGAATCTATCGCCTATTATTAAGCTTCGTAATTTTAACAATGCGATTAAGTATATGTTAATTGACAAATATACAAAACCTGGAGATGTCGTTTTAGAACTCGGATGTGGCAAAGGTGGTGATTTAAGAAAATATGGTGCTGCAGGTATTTCACAGTTTATTGGTATTGACATTTCCAATGCCTCTATCCAAGAAGCTCATAAGAGATACCGGTCCATGAGAAATTTGGACTACCAAGTTGTGCTAATTACTGGGGATTGCTTTGGCGAATCATTAGGTGTTGCAGTAGAGCCCTTCCCAGACTGTAGATTTCCGTGTGATATCGTTTCGACGCAATTTTGCTTGCATTATGCTTTTGAGACCGAAGAAAAAGCAAGGAGAGCTTTACTGAATGTCGCCAAATCACTCAAAATTGGTGGTCACTTTTTTGGCACGATACCAGATTCAGAATTCATTCGTTATAAACTGAATAAATTTCCCAAAGAAGTGGAAAAGCCATCTTGGGGAAACTCCATTTATAAAGTGACATTTGAGAATAATTCATACCAAAAGAATGATTATGAGTTCACATCGCCTTATGGTCAAATGTATACTTATTGGCTAGAAGATGCTATAGATAATGTTCCTGAATATGTTGTGCCATTCGAAACCTTAAGAAGTTTGGCTGATGAATACGGTTTGGAACTAGTATCGCAAATGCCTTTTAATAAATTTTTTGTCCAAGAAATTCCCAAATGGATTGAGAGGTTTTCGCCTAAAATGAGGGAAGGTTTACAAAGATCTGATGGTAGATATGGTGTTGAAGGGGATGAAAAGGAAGCGGCATCATATTTCTATACCATGTTTGCATTCCGTAAGGTAAAACAGTATATCGAACCGGAAAGCGTAAAGCCCAACTGAGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAA

Vector Information:

Vector Name:pDONR201
Type:bacterial plasmid
Description:Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Properties:Gateway, donor (entry), recombinational cloning
Comments:The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Map:pdonr201_pdonr207_map.pdf
Sequence:pDONR201_FASTA.txt
Size (bp):4470
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha T1 phage resistant Y

Antibiotic Selections:

Host Type Marker
bacterial kanamycin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 50ug/mL kanamycin Growth with the single antibiotic in LB at 37 degrees is recommended. Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Authors:

Author Name Author Type
HIP Academic Institute

Publications:

PMID Title
17322287 Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.