Clone: EvNO00017260 ← Click Here To Order

Clone ID:EvNO00017260
Is Verified:Y
Status:AVAILABLE
Source:Steve Elledge
Description:Lentiviral expression vector for shRNA expression with inducible (TREX) promoter, fluorescent (GFP marker); amp resistance; MAGIC bacterial mating cloning.
Comments:This is an 'empty vector' version pPRIME for MAGIC bacterial mating-based strategy (see PMID 15731760)
Type:No insert
Verification Method:Sequence Verification
Distribution:Academic and non-profit labs
Special MTA:
Map:p192.JPG

Related Identifiers:

SYNONYM p192
SYNONYM pPRIME-TREX-GFP-recipient
SYNONYM pPRIME-TREX-GFP-PheS-recipient

Vector Information:

Vector Name:pPRIME-TREX-GFP-recipient
Type:bacterial plasmid
Description:Lentiviral expression vector for shRNA expression with inducible (TREX) promoter, fluorescent (GFP marker); amp resistance; MAGIC bacterial mating cloning.
Properties:MAGIC mating system, RNA interference, fluorescent marker, inducible expression, lentiviral infection, mammalian expression, mammalian transduction, retroviral vector, with tag/fusion/marker
Comments:MAGIC mating recipient for PRIME approach (Elledge Lab)
Map:p192.JPG
Sequence:p192.txt
Size (bp):8724
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
BW28705I/pML300 N Mating recipient host strain in LB/Sp50/Cb100/0.2% glycose at 30 degrees C (not 37 degrees C)
EB5-alpha T1, T5 phage resistant Y EB5-alpha host as supplied by Edge BioSystems

Antibiotic Selections:

Host Type Marker
bacterial ampicillin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type
Steve Elledge Clone PI
Frank Stegmeier Clone first author and donor

Publications:

PMID Title
16141338 A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells
15731760 MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules