Clone: EvNO00017259 ← Click Here To Order
| Clone ID: | EvNO00017259 |
| Is Verified: | Y |
| Status: | AVAILABLE |
| Source: | Steve Elledge |
| Description: | Lentiviral expression vector for shRNA expression with cell-surface marker; amp resistance; MAGIC bacterial mating cloning. |
| Comments: | This is an 'empty vector' version pPRIME for MAGIC bacterial mating-based strategy (see PMID 15731760) |
| Type: | No insert |
| Verification Method: | Sequence Verification |
| Distribution: | Academic and non-profit labs |
| Special MTA: | |
Related Identifiers:
Vector Information:
| Vector Name: | pPRIME-CMV-LNGFR-recipient |
| Type: | bacterial plasmid |
| Description: | Lentiviral expression vector for shRNA expression with cell-surface marker; amp resistance; MAGIC bacterial mating cloning. |
| Properties: | MAGIC mating system, RNA interference, lentiviral infection, mammalian expression, mammalian transduction, retroviral vector |
| Comments: | MAGIC mating recipient for PRIME approach (Elledge Lab) |
| Map: | p211.JPG |
| Sequence: | p211.txt |
| Size (bp): | 8744 |
| Form: | dsDNA |
Host Information:
| Host Strain |
Is Used In Distribution |
Description |
| BW28705I/pML300 |
N |
Mating recipient host strain in LB/Sp50/Cb100/0.2% glycose at 30 degrees C (not 37 degrees C) |
| EB5-alpha T1, T5 phage resistant |
Y |
EB5-alpha host as supplied by Edge BioSystems |
Antibiotic Selections:
| Host Type |
Marker |
| bacterial |
ampicillin |
Recommended Growth Condition:
| Host Type |
Selection Condition |
Growth Condition |
Comments |
| bacterial |
100 ug/mL ampicillin |
Commonly used conditions for ampicillin resistant plasmid clones. |
Authors:
| Author Name |
Author Type |
| Steve Elledge |
Clone PI |
| Frank Stegmeier |
Clone first author and donor |
Publications:
| PMID |
Title |
| 16141338 |
A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells |
| 15731760 |
MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules |
