Clone ID:	EvNO00017259
Is Verified:	Y
Status:	AVAILABLE
Source:	Steve Elledge
Description:	Lentiviral expression vector for shRNA expression with cell-surface marker; amp resistance; MAGIC bacterial mating cloning.
Comments:	This is an 'empty vector' version pPRIME for MAGIC bacterial mating-based strategy (see PMID 15731760)
Type:	No insert
Verification Method:	Sequence Verification
Distribution:	Academic and non-profit labs
Special MTA:

Map:

p211.JPG

Related Identifiers:

SYNONYM	p211
SYNONYM	pPRIME-CMV-LNGFR-recipient
SYNONYM	pPRIME-CMV-NGFR-PheS-recipient

Vector Information:

Vector Name:	pPRIME-CMV-LNGFR-recipient
Type:	bacterial plasmid
Description:	Lentiviral expression vector for shRNA expression with cell-surface marker; amp resistance; MAGIC bacterial mating cloning.
Properties:	MAGIC mating system, RNA interference, lentiviral infection, mammalian expression, mammalian transduction, retroviral vector
Comments:	MAGIC mating recipient for PRIME approach (Elledge Lab)
Map:	p211.JPG
Sequence:	p211.txt
Size (bp):	8744
Form:	dsDNA

Host Information:

Host Strain	Is Used In Distribution	Description
BW28705I/pML300	N	Mating recipient host strain in LB/Sp50/Cb100/0.2% glycose at 30 degrees C (not 37 degrees C)
EB5-alpha T1, T5 phage resistant	Y	EB5-alpha host as supplied by Edge BioSystems

Antibiotic Selections:

Host Type	Marker
bacterial	ampicillin

Recommended Growth Condition:

Host Type	Selection Condition	Growth Condition	Comments
bacterial	100 ug/mL ampicillin	Growth with the single antibiotic in LB at 37 degrees is recommended.	Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name	Author Type
Steve Elledge	Clone PI
Frank Stegmeier	Clone first author and donor

Publications:

PMID	Title
16141338	A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells
15731760	MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules