Related Identifiers:
Vector Information:
Vector Name: | pPRIME-CMV-LNGFR-recipient |
Type: | bacterial plasmid |
Description: | Lentiviral expression vector for shRNA expression with cell-surface marker; amp resistance; MAGIC bacterial mating cloning. |
Properties: | MAGIC mating system, RNA interference, lentiviral infection, mammalian expression, mammalian transduction, retroviral vector |
Comments: | MAGIC mating recipient for PRIME approach (Elledge Lab) |
Map: | p211.JPG |
Sequence: | p211.txt |
Size (bp): | 8744 |
Form: | dsDNA |
Host Information:
Host Strain |
Is Used In Distribution |
Description |
BW28705I/pML300 |
N |
Mating recipient host strain in LB/Sp50/Cb100/0.2% glycose at 30 degrees C (not 37 degrees C) |
EB5-alpha T1, T5 phage resistant |
Y |
EB5-alpha host as supplied by Edge BioSystems |
Antibiotic Selections:
Host Type |
Marker |
bacterial |
ampicillin |
Recommended Growth Condition:
Host Type |
Selection Condition |
Growth Condition |
Comments |
bacterial |
100 ug/mL ampicillin |
Commonly used conditions for ampicillin resistant plasmid clones. |
Authors:
Author Name |
Author Type |
Steve Elledge |
Clone PI |
Frank Stegmeier |
Clone first author and donor |
Publications:
PMID |
Title |
16141338 |
A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells |
15731760 |
MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules |