Clone: EvNO00013285
| Clone ID: | EvNO00013285 |
| Is Verified: | U |
| Status: | AVAILABLE |
| Source: | James DeCaprio |
| Description: | Eukaryotic expression vector (unknown promoter); amp resistance (puromycin for mammalian selection); restriction enzyme cloning. |
| Comments: | |
| Type: | No insert |
| Verification Method: | |
| Distribution: | Academic and non-profit labs |
| Special MTA: |
Related Identifiers:
| SYNONYM | pE-puro |
| SYNONYM | DeCaprio_21 |
| SYNONYM | pEpuro |
Sequencing Information:
| Phred Score: | 913 |
| Date: | 2019-03-20 |
Last Passing Sequencing Read:
GTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGAATTCAAGCTTACCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCAGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGATCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGACGCCCGCCCCACGACCCGCAGCGCCCGACCGAAAGGAGCGCACGACCCCATGCATCGAGCTTGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGC
Vector Information:
| Vector Name: | pEpuro |
| Type: | bacterial plasmid |
| Description: | Eukaryotic expression vector (unknown promoter); amp resistance (puromycin for mammalian selection); restriction enzyme cloning. |
| Properties: | mammalian expression |
| Comments: | |
| Map: | |
| Sequence: | |
| Size (bp): | 0 |
| Form: | dsDNA |
Host Information:
| Host Strain | Is Used In Distribution | Description |
| EB5-alpha T1, T5 phage resistant | Y | EB5-alpha host as supplied by Edge BioSystems |
Antibiotic Selections:
| Host Type | Marker |
| bacterial | ampicillin |
| mammalian | puromycin |
Recommended Growth Condition:
| Host Type | Selection Condition | Growth Condition | Comments |
| bacterial | 100 ug/mL ampicillin | Growth with the single antibiotic in LB at 37 degrees is recommended. | Commonly used conditions for ampicillin resistant plasmid clones. |
Authors:
| Author Name | Author Type |
| James DeCaprio | Clone PI |
| Jocelyn Kasper | Clone donor |
Publications:
| PMID | Title |
| 7565733 | Role of pRb-related proteins in simian virus 40 large-T-antigen-mediated transformation |
