Clone: ScCD00102963

Clone ID:ScCD00102963
Is Verified:N
Status:AVAILABLE
Source:HIP
Description:
Comments:
Type:cDNA
Verification Method:Insert was fully sequenced in parent vector
Distribution:No restriction
Special MTA:
Map:

Related Identifiers:

HIP Clone ID 469432
HIP Master Clone ID 177093
Original Clone ID FLH469432.01X

Insert Information:

Insert Size (bp) Species Format Tissue Source Species Specific ID Gene Symbol Gene Name Keyword
1 1569 Saccharomyces cerevisiae CLOSED Genomic DNA template YCL043C PDI1 Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, essential for the formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds
Insert1
Size (bp)1569
SpeciesSaccharomyces cerevisiae
FormatCLOSED
Tissue SourceGenomic DNA template
Species Specific ID YCL043C
Gene SymbolPDI1
Gene NameProtein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, essential for the formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds
Keyword

Insert Sequence:

ATGAAGTTTTCTGCTGGTGCCGTCCTGTCATGGTCCTCCCTGCTGCTCGCCTCCTCTGTTTTCGCCCAACAAGAGGCTGTGGCCCCTGAAGACTCCGCTGTCGTTAAGTTGGCCACCGACTCCTTCAATGAGTACATTCAGTCGCACGACTTGGTGCTTGCGGAGTTTTTTGCTCCATGGTGTGGCCACTGTAAGAACATGGCTCCTGAATACGTTAAAGCCGCCGAGACTTTAGTTGAGAAAAACATTACCTTGGCCCAGATCGACTGTACTGAAAACCAGGATCTGTGTATGGAACACAACATTCCAGGGTTCCCAAGCTTGAAGATTTTCAAAAACAGCGATGTTAACAACTCGATCGATTACGAGGGACCTAGAACTGCCGAGGCCATTGTCCAATTCATGATCAAGCAAAGCCAACCGGCTGTCGCCGTTGTTGCTGATCTACCAGCTTACCTTGCTAACGAGACTTTTGTCACTCCAGTTATCGTCCAATCCGGTAAGATTGACGCCGACTTCAACGCCACCTTTTACTCCATGGCCAACAAACACTTCAACGACTACGACTTTGTCTCCGCTGAAAACGCAGACGATGATTTCAAGCTTTCTATTTACTTGCCCTCCGCCATGGACGAGCCTGTAGTATACAACGGTAAGAAAGCCGATATCGCTGACGCTGATGTTTTTGAAAAATGGTTGCAAGTGGAAGCCTTGCCCTACTTTGGTGAAATCGACGGTTCCGTTTTCGCCCAATACGTCGAAAGCGGTTTGCCTTTGGGTTACTTATTCTACAATGACGAGGAAGAATTGGAAGAATACAAGCCTCTCTTTACCGAGTTGGCCAAAAAGAACAGAGGTCTAATGAACTTTGTTAGCATCGATGCCAGAAAATTCGGCAGACACGCCGGCAACTTGAACATGAAGGAACAATTCCCTCTATTTGCCATCCACGACATGACTGAAGACTTGAAGTACGGTTTGCCTCAACTCTCTGAAGAGGCGTTTGACGAATTGAGCGACAAGATCGTGTTGGAGTCTAAGGCTATTGAATCTTTGGTTAAGGACTTCTTGAAAGGTGATGCCTCCCCAATCGTGAAGTCCCAAGAGATCTTCGAGAACCAAGATTCCTCTGTCTTCCAATTGGTCGGTAAGAACCATGACGAAATCGTCAACGACCCAAAGAAGGACGTTCTTGTTTTGTACTATGCCCCATGGTGTGGTCACTGTAAGAGATTGGCCCCAACTTACCAAGAACTAGCTGATACCTACGCCAACGCCACATCCGACGTTTTGATTGCTAAACTAGACCACACTGAAAACGATGTCAGAGGCGTCGTAATTGAAGGTTACCCAACAATCGTCTTATACCCAGGTGGTAAGAAGTCCGAATCTGTTGTGTACCAAGGTTCAAGATCCTTGGACTCTTTATTCGACTTCATCAAGGAAAACGGTCACTTCGACGTCGACGGTAAGGCCTTGTACGAAGAAGCCCAGGAAAAAGCTGCTGAGGAAGCCGATGCTGACGCTGAATTGGCTGACGAAGAAGATGCCATTCACGATGAATTGTAG

Insert Property: Insert 1

Type Value Extra Information
End on reference sequence 1569
Start on reference sequence 1

Inferred Whole Plasmid Sequence: (BETA)
Insert green, Vector black

ATTGTCATTGAACTTGCCTGAAGAAGGTAAGGCCATTGAAGATGCAGTTAAAAAGGTTTTGGATGCAGGTATCAGAACTGGTGATTTAGGTGGTTCCAACAGTACCACCGAAGTCGGTGATGCTGTCGCCGAAGAAGTTAAGAAAATCCTTGCTTAAAAAGATTCTCTTTTTTTATGATATTTGTACATAAACTTTATAAATGAAATTCATAATAGAAACGACACGAAATTACAAAATGGAATATGTTCATAGGGTAGAATTAATTCTCATGTTTGACAGCTTATCATCGGATCGATCCAATATCAAAGGAAATGATAGCATTGAAGGATGAGACTAATCCAATTGAGGAGTGGCAGCATATAGAACAGCTAAAGGGTAGTGCTGAAGGAAGCATACGATACCCCGCATGGAATGGGATAATATCACAGGAGGTACTAGACTACCTTTCATCCTACATAAATAGACGCATATAAGTACGCATTTAAGCATAAACACGCACTATGCCGTTCTTCTCATGTATATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTGTATGTGCGCAGCTCGCGTTGCATTTTCGGAAGCGCTCGTTTTCGGAAACGCTTTGAAGTTCCTATTCCGAAGTTCCTATTCTCTAGCTAGAAAGTATAGGAACTTCAGAGCGCTTTTGAAAACCAAAAGCGCTCTGAAGACGCACTTTCAAAAAACCAAAAACGCACCGGACTGTAACGAGCTACTAAAATATTGCGAATACCGCTTCCACAAACATTGCTCAAAAGTATCTCTTTGCTATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGCATCTAAACTCGACCTCTACATTTTTTATGTTTATCTCTAGTATTACTCTTTAGACAAAAAAATTGTAGTAAGAACTATTCATAGAGTGAATCGAAAACAATACGAAAATGTAAACATTTCCTATACGTAGTATATAGAGACAAAATAGAAGAAACCGTTCATAATTTTCTGACCAATGAAGAATCATCAACGCTATCACTTTCTGTTCACAAAGTATGCGCAATCCACATCGGTATAGAATATAATCGGGGATGCCTTTATCTTGAAAAAATGCACCCGCAGCTTCGCTAGTAATCAGTAAACGCGGGAAGTGGAGTCAGGCTTTTTTTATGGAAGAGAAAATAGACACCAAAGTAGCCTTCTTCTAACCTTAACGGACCTACAGTGCAAAAAGTTATCAAGAGACTGCATTATAGAGCGCACAAAGGAGAAAAAAAGTAATCTAAGATGCTTTGTTAGAAAAATAGCGCTCTCGGGATGCATTTTTGTAGAACAAAAAAGAAGTATAGATTCTTTGTTGGTAAAATAGCGCTCTCGCGTTGCATTTCTGTTCTGTAAAAATGCAGCTCAGATTCTTTGTTTGAAAAATTAGCGCTCTCGCGTTGCATTTTTGTTTTACAAAAATGAAGCACAGATTCTTCGTTGGTAAAATAGCGCTTTCGCGTTGCATTTCTGTTCTGTAAAAATGCAGCTCAGATTCTTTGTTTGAAAAATTAGCGCTCTCGCGTTGCATTTTTGTTCTACAAAATGAAGCACAGATGCTTCGTTAACAAAGATATGCTATTGAAGTGCAAGATGGAAACGCAGAAAATGAACCGGGGATGCGACGTGCAAGATTACCTATGCAATAGATGCAATAGTTTCTCCAGGAACCGAAATACATACATTGTCTTCCGTAAAGCGCTAGACTATATATTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGTTCGGATGTTCAAAATTCAATGATGGGTAACAAGTACGATCCGATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGGTACCGCGGCCGCTCTAATGGCTTTGCTAATTGCTGATTGAAAGATATATGACCAATTAACTTGATGATTGCTATATGCGCGTTCTATTGAAGCAACGTGCAAGATGATCACTGCTTGGATTGACCTTGTTTTAGCAACAAGCCATACAAAGATATCAGACAGGAAAGTCACAGTCAAAGCATGCAAAAATCGACAAAGCAAACATAAGTAGAACCTGAGAACATCAACCATATGTCCTAATGAATTCAAGCACCCTCTTTTCCACCCAATTTGAGACATTTTCAAGATGTATTTATATTTTCGTGTACTGTGATATGACTTTATTCTTAGAATTTTTTATTGCCCGCGTAAGAAGGTGTTTAACAAGAGATCTTGTTAACTCACAAGGGTACTCTGACTGGATAGAAATCTTATTCTTGACCGGAAACTATCGAACTACAAAGAAAGAGCTTAGTTAATACAAATAAAAAAGTACAATGGAGCTCGGTAAACCTATTCCTAATCCTCTCCTAGGTTTAGATTCTACTGCTGCCGGCAAGCCCATCCCCAACCCCTTGCTTGGCTTGGACTCCACCGCCGCAGGAAAACCAATACCAAATCCACTTCTCGGACTTGATTCAACAACTAGTCATGTAGGATCAACAAGTTTGTACAAAAAAGCAGGCTCCACCATGAAGTTTTCTGCTGGTGCCGTCCTGTCATGGTCCTCCCTGCTGCTCGCCTCCTCTGTTTTCGCCCAACAAGAGGCTGTGGCCCCTGAAGACTCCGCTGTCGTTAAGTTGGCCACCGACTCCTTCAATGAGTACATTCAGTCGCACGACTTGGTGCTTGCGGAGTTTTTTGCTCCATGGTGTGGCCACTGTAAGAACATGGCTCCTGAATACGTTAAAGCCGCCGAGACTTTAGTTGAGAAAAACATTACCTTGGCCCAGATCGACTGTACTGAAAACCAGGATCTGTGTATGGAACACAACATTCCAGGGTTCCCAAGCTTGAAGATTTTCAAAAACAGCGATGTTAACAACTCGATCGATTACGAGGGACCTAGAACTGCCGAGGCCATTGTCCAATTCATGATCAAGCAAAGCCAACCGGCTGTCGCCGTTGTTGCTGATCTACCAGCTTACCTTGCTAACGAGACTTTTGTCACTCCAGTTATCGTCCAATCCGGTAAGATTGACGCCGACTTCAACGCCACCTTTTACTCCATGGCCAACAAACACTTCAACGACTACGACTTTGTCTCCGCTGAAAACGCAGACGATGATTTCAAGCTTTCTATTTACTTGCCCTCCGCCATGGACGAGCCTGTAGTATACAACGGTAAGAAAGCCGATATCGCTGACGCTGATGTTTTTGAAAAATGGTTGCAAGTGGAAGCCTTGCCCTACTTTGGTGAAATCGACGGTTCCGTTTTCGCCCAATACGTCGAAAGCGGTTTGCCTTTGGGTTACTTATTCTACAATGACGAGGAAGAATTGGAAGAATACAAGCCTCTCTTTACCGAGTTGGCCAAAAAGAACAGAGGTCTAATGAACTTTGTTAGCATCGATGCCAGAAAATTCGGCAGACACGCCGGCAACTTGAACATGAAGGAACAATTCCCTCTATTTGCCATCCACGACATGACTGAAGACTTGAAGTACGGTTTGCCTCAACTCTCTGAAGAGGCGTTTGACGAATTGAGCGACAAGATCGTGTTGGAGTCTAAGGCTATTGAATCTTTGGTTAAGGACTTCTTGAAAGGTGATGCCTCCCCAATCGTGAAGTCCCAAGAGATCTTCGAGAACCAAGATTCCTCTGTCTTCCAATTGGTCGGTAAGAACCATGACGAAATCGTCAACGACCCAAAGAAGGACGTTCTTGTTTTGTACTATGCCCCATGGTGTGGTCACTGTAAGAGATTGGCCCCAACTTACCAAGAACTAGCTGATACCTACGCCAACGCCACATCCGACGTTTTGATTGCTAAACTAGACCACACTGAAAACGATGTCAGAGGCGTCGTAATTGAAGGTTACCCAACAATCGTCTTATACCCAGGTGGTAAGAAGTCCGAATCTGTTGTGTACCAAGGTTCAAGATCCTTGGACTCTTTATTCGACTTCATCAAGGAAAACGGTCACTTCGACGTCGACGGTAAGGCCTTGTACGAAGAAGCCCAGGAAAAAGCTGCTGAGGAAGCCGATGCTGACGCTGAATTGGCTGACGAAGAAGATGCCATTCACGATGAATTGTAGGACCCAGCTTTCTTGTACAAAGTGGTTGATCCTATCTAGTTAGGTGAGTAAACAGGCCCCTTTTCCTTTGTCGATATCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCTCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTTAATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAAACGCGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAATTAACTGTGGGAATACTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGCAACCATTATTTTTTTCCTCAACATAACGAGAACACACAGGGGCGCTATCGCACAGACAAATTCGATGACTGGAAATTTTTTGTTAATTTCAGAGGTCGCCGCGCATATACCTTTTTCAACTGAAAAATTGGGAGAAAAAGGAAAGGTGAGAGGCCGGAACCGGCTTTTCATATAGAATAGAGAAGCGTTCATGACTAAATGCTTGCATCACAATACTTGAAGTTGACAATATTATTTAAGGACCTATTGTTTTTTCCAATAGGTGGTTAGCAATCGTCTTATTCTAACTTTTCTTACCTTTTACATTTCAGCAATATATATATATATTTCAAGGATATACATTCTAATGTCTGCCCCTATGTCTGCCCCTAAGAAGATCGTCGTTTTGCCAGGTGACCACGTTGGTCAAGATCACAGCCGAAGCCATTAAGGTTCTTAAAGCTATTTCTGATGTTCGTTCCAATGTCAAGTTCGATTTCGAAAATCATTTAATTGGTGGTGCTGCTATCGATGCTACAGGTGTCCCACTTCCAGATGAGGCGCTGGAAGCCTCCAAGAAGGTTGATGCCGTTTTGTTAGGTGCTGTGGGTGGTCCTAAATGGGGTACAGGTAGTGTTAGACCTGAACAAGGTTTACTAAAAATCCGTAAAGAACTTCAATTGTACGCCAACTTAAGACCATGTAACTTTGCATCCGACTCTCTTTTAGACTTATCTCCAATCAAGCCACAATTTGCTAAAGGTACTGACTTCGTTGTTGTCAGAGAATTAGTGGGAGGTATTTACTTTGGTAAGAGAAAGGAAGACGATGGTGATGGTGTCGCTTGGGATAGTGAACAATACACCGTTCCAGAAGTGCAAAGAATCACAAGAATGGCCGCTTTCATGGCCCTACAACATGAGCCACCATTGCCTATTTGGTCCTTGGATAAAGCTAATGTTTTGGCCTCTTCAAGATTATGGAGAAAAACTGTGGAGGAAACCATCAAGAACGAATTTCCTACATTGAAGGTTCAACATCAATTGATTGATTCTGCCGCCATGATCCTAGTTAAGAACCCAACCCACCTAAATGGTATTATAATCACCAGCAACATGTTTGGTGATATCATCTCCGATGAAGCCTCCGTTATCCCAGGTTCCTTGGGTTTGTTGCCATCTGCGTCCTTGGCCTCTTTGCCAGACAAGAACACCGCATTTGGTTTGTACGAACCATGCCACGGTTCTGCTCCAGATTTGCCAAAGAATAAGGTTGACCCTATCGCCACTATCTTGTCTGCTGCAATGATGTTGAA

Vector Information:

Vector Name:ZM552
Type:bacterial plasmid
Description:Yeast expression vectorn with N-terminal 3X V5 epitope tag; ampicillin and chloramphenicol resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
Properties:Gateway, with tag/fusion/marker, yeast expression
Comments:ZM511 Stu1 vector plus Gateway attR frame B cassette EcoRV fragment; same as ZM515
Map:
Sequence:ZM552.txt
Size (bp):8302
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha T1 phage resistant Y phage-resistant E. coli DH5-alpha strain used by HIP

Antibiotic Selections:

Host Type Marker
bacterial ampicillin
yeast LEU2

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.