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Vector: pWALIUM20

Name: pWALIUM20
Description: Drosophila expression vector. loxP, and GATEWAY recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter with ftz intron and SV40 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 10540
Properties: drosophila in vitro and in vivo expression
Vector Map: WALIUM20_map_seq.pdf
Vector Sequence: pWALIUM20.txt
Comments: Revised 4/25/2012

Features:

Type Name Description Start Position End Position
bacterial origin F1 origin 138 444
bacterial operon LacO Alpha 463 531
primer site M13 forward 602 618
promoter T7 618 645
selectable marker white 682 4808
Cloning Site attB1 4851 5218
repressor binding site Gypsy Insulator 5230 5660
Cloning Site LoxP 5678 5711
enhancer 5xUAS UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription 5718 5827
Cloning Site LoxP #2 5834 5867
enhancer 5xUAS #2 UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription 5874 5983
promoter Hsp70 70 kilodalton heat shock protein 5990 6249
primer site pUAST_Forward 6177 6193
restriction site Nhe I 6301 6306
primer site M13 Forward #2 6332 6348
restriction site EcoRI 6918 6923
intron ftz Involved in DNA binding. Also known as CG2047-PA,Fushi-Tarazu, Ultra-abdominal-like, ftz-PA, fushi tarazu 7030 7176
poly-A signal SV40 7200 7899
repressor binding site Gypsy Insulator #2 7906 8336
primer site T3 8352 8371
primer site M13 reverse 8389 8409
bacterial origin ColE1 8777 9459
bacterial selection antibiotic AmpR 9558 10217

Authors:

Author Name Author Type Creation Date
Norbert Perrimon Academic researcher, vector PI
Jian-Quan Ni Academic researcher, vector creator

Publications:

PMID Title

Parent Vector:

Name Comments