Vector: pVALIUM10-moe

Name: pVALIUM10-moe
Description: Drosophila expression vector for Multicloning site, Over-Expression (MOE). loxP sites and restriction sites for EcoRI, BgIII, and Xbal.Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, MCS, with ftz intron and SV40 polyA. Vermilion selection in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 7541
Properties: drosophila in vitro and in vivo expression
Vector Map: VALIUM10moe_map_seq.pdf
Vector Sequence: pVALIUM10-moe.txt
Comments: Revised 4/25/2012

Features:

Type Name Description Start Position End Position
bacterial origin F1 origin 138 444
bacterial operon LacZ Alpha 463 531
primer site M13 forward 602 619
primer site T7 618 645
selectable marker vermilion 664 2543
Cloning Site attB1 2568 2935
repressor binding site Gypsy Insulator 2947 3377
Cloning Site LoxP 3395 3428
enhancer 5xUAS UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription 3435 3544
Cloning Site LoxP #2 3551 3584
enhancer 5xUAS #2 UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription 3591 3700
promoter Hsp70 70 kilodalton heat shock protein 3707 3966
primer site pUAST_Forward TCAACTGCAACTACTGA hsp70(+60)f Recommended for insert verification 3894 3910
Cloning Site MCS 3967 3990
intron ftz Involved in DNA binding. Also known as CG2047-PA,Fushi-Tarazu, Ultra-abdominal-like, ftz-PA, fushi tarazu 4031 4177
polyA site SV40 4201 4900
repressor binding site Gypsy Insulator #2 4907 5337
primer site T3 5354 5373
primer site M13 reverse 5391 5411
bacterial operon LacO 5417 5439
bacterial origin ColE1 5779 6461
bacterial selection antibiotic AmpR 6559 7218

Authors:

Author Name Author Type Creation Date
Norbert Perrimon Academic researcher, vector PI
Jian-Quan Ni Academic researcher, vector creator

Publications:

PMID Title

Parent Vector:

Name Comments