Clone: EvNO00061632

Clone ID:EvNO00061632
Is Verified:U
Status:NOT AVAILABLE
Source:Mekalanos lab
Description:Vector (in DH5a lambda pir) for transposon mediated mutagenesis of bacteria, with promoterless and signal sequence-less bla gene in transposon such that only fusion to transmembrane or secreted gene results in amp resistance; amp resistance; restriction enzyme cloning.
Comments:Clone does not grow or cannot be found.
Type:No insert
Verification Method:
Distribution:Academic and non-profit labs
Special MTA:
Map:

Related Identifiers:

SYNONYM pWL10 in DH5-alpha lambda-pir
Original Clone ID pWL10 (in DH5a)
SYNONYM pWL10
SYNONYM Mekalanos Strain C26

Property:

Insert Information:

Insert Size (bp) Species Format Tissue Source Species Specific ID Gene Symbol Gene Name Target Genbank Keyword

Insert Sequence:

Vector Information:

Vector Name:pWL10
Type:bacterial plasmid
Description:Vector for transposon mediated mutagenesis of bacteria, with promoterless and signal sequence-less bla gene (ampR) in transposon such that only fusion to transmembrane or secreted gene results in ampicillin resistance; ampicillin resistance; restriction enzyme cloning.
Properties:transposon mediated gene disruption
Comments:
Map:
Sequence:
Size (bp):0
Form:dsDNA

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha lambda-pir Y E. coli host strain DH5-alpha lambda-pir

Antibiotic Selections:

Host Type Marker
bacterial ampicillin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type
John Mekalanos Academic researcher, clone PI
Su Chiang Academic researcher, clone donor

Publications:

PMID Title
9435261 TcpP protein is a positive regulator of virulence gene expression in Vibrio cholerae