Clone: EvNO00061632

Clone ID: EvNO00061632 Type: No insert
Is Verified: U Verification Method:
Status: NOT AVAILABLE Distribution: Academic and non-profit labs
Source: Mekalanos lab Special MTA:
Description: Vector (in DH5a lambda pir) for transposon mediated mutagenesis of bacteria, with promoterless and signal sequence-less bla gene in transposon such that only fusion to transmembrane or secreted gene results in amp resistance; amp resistance; restriction enzyme cloning.
Comments: Clone does not grow or cannot be found.
Map:

Related Identifiers:

SYNONYM pWL10 in DH5-alpha lambda-pir
Original Clone ID pWL10 (in DH5a)
SYNONYM pWL10
SYNONYM Mekalanos Strain C26

Property:

Insert Information:

Insert Size (bp) Species Mutation Discrepancy Format Tissue Source Species Specific ID Gene Symbol Gene Name Target Genbank Keyword

Insert Sequence:

Vector Information:

Vector Name: pWL10 Size (bp): 0
Type: bacterial plasmid Form: dsDNA
Description: Vector for transposon mediated mutagenesis of bacteria, with promoterless and signal sequence-less bla gene (ampR) in transposon such that only fusion to transmembrane or secreted gene results in ampicillin resistance; ampicillin resistance; restriction enzyme cloning.
Properties: transposon mediated gene disruption
Comments:
Map:
Sequence:

Host Information:

Host Strain Is Used In Distribution Description
DH5-alpha lambda-pir Y E. coli host strain DH5-alpha lambda-pir

Antibiotic Selections:

Host Type Marker
bacterial ampicillin

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type
John Mekalanos Academic researcher, clone PI
Su Chiang Academic researcher, clone donor

Publications:

PMID Title
9435261 TcpP protein is a positive regulator of virulence gene expression in Vibrio cholerae