Clone: EvNO00422224

Clone ID: EvNO00422224 Type: No insert
Is Verified: N Verification Method:
Status: AVAILABLE Distribution: Academic and non-profit labs
Source: DRSC Special MTA:
Description: Drosophila expression vector for high expression of hairpin insert. loxP, and GATEWAY recombination sites, NheI andEcRI method to clone shmiR. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html P-Trans promoter with ftz intron, two gypsy insulators,two 5xUAS, K10 localization element and vermilion selection in drosophila. AmpR in bacteria; vermilion marker in drosophila. Specialty sequencing primer required to verify insert. Vector may recombine in bacteria and should be stored as DNA.
Comments:
Map: VALIUM22-map-seq.pdf

Related Identifiers:

Property:

Insert Information:

Insert Size (bp) Species Mutation Discrepancy Format Tissue Source Species Specific ID Gene Symbol Gene Name Target Genbank Keyword

Insert Sequence:

Vector Information:

Vector Name: pVALIUM22 Size (bp): 8973
Type: bacterial plasmid Form: dsDNA
Description: Drosophila expression vector for high expression of hairpin insert. loxP, and GATEWAY recombination sites, NheI andEcRI method to clone shmiR. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html P-Trans promoter with ftz intron, two gypsy insulators,two 5xUAS, K10 localization element and vermilion selection in drosophila. AmpR in bacteria; vermilion marker in drosophila. Specialty sequencing primer required to verify insert. Vector may recombine in bacteria and should be stored as DNA.
Properties: drosophila in vitro and in vivo expression
Comments: Revised 4/25/2012
Map: VALIUM22_map_seq.pdf
Sequence: pVALIUM22.txt

Host Information:

Host Strain Is Used In Distribution Description
EB5-alpha N

Antibiotic Selections:

Host Type Marker
bacterial ampicillin
drosophila vermilion

Recommended Growth Condition:

Host Type Selection Condition Growth Condition Comments
bacterial 100 ug/mL ampicillin Growth with the single antibiotic in LB at 37 degrees is recommended. Commonly used conditions for ampicillin resistant plasmid clones.

Authors:

Author Name Author Type

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PMID Title